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Successful transmission electron microscopy in all of its manifestations depends on the quality of the specimens examined. Biological specimen preparation protocols have usually been more rigorous and time consuming than those in the physical sciences. For this reason, there has been a wealth of scienti c literature detailing speci c preparation steps and numerous excellent books on the preparation of b- logical thin specimens. This does not mean to imply that physical science specimen preparation is trivial. For the most part, most physical science thin specimen pre- ration protocols can be executed in a matter of a few hours using straightforward steps. Over the years, there has been a steady stream of papers written on various aspects of preparing thin specimens from bulk materials. However, aside from s- eral seminal textbooks and a series of book compilations produced by the Material Research Society in the 1990s, no recent comprehensive books on thin specimen preparation have appeared until this present work, rst in French and now in English. Everyone knows that the data needed to solve a problem quickly are more imp- tant than ever. A modern TEM laboratory with supporting SEMs, light microscopes, analytical spectrometers, computers, and specimen preparation equipment is an investment of several million US dollars. Fifty years ago, electropolishing, chemical polishing, and replication methods were the principal specimen preparation me- ods.
List of contents
Techniques: General Introduction.- Preliminary Preparation Techniques.- Thinning Preparation Techniques.- Mechanical Preparation Techniques.- Replica Techniques.- Techniques Specific to Fine Particles.- Contrast Enhancement and Labeling Techniques.
Summary
Successful transmission electron microscopy in all of its manifestations depends on the quality of the specimens examined. Biological specimen preparation protocols have usually been more rigorous and time consuming than those in the physical sciences. For this reason, there has been a wealth of scienti c literature detailing speci c preparation steps and numerous excellent books on the preparation of b- logical thin specimens. This does not mean to imply that physical science specimen preparation is trivial. For the most part, most physical science thin specimen pre- ration protocols can be executed in a matter of a few hours using straightforward steps. Over the years, there has been a steady stream of papers written on various aspects of preparing thin specimens from bulk materials. However, aside from s- eral seminal textbooks and a series of book compilations produced by the Material Research Society in the 1990s, no recent comprehensive books on thin specimen preparation have appeared until this present work, rst in French and now in English. Everyone knows that the data needed to solve a problem quickly are more imp- tant than ever. A modern TEM laboratory with supporting SEMs, light microscopes, analytical spectrometers, computers, and specimen preparation equipment is an investment of several million US dollars. Fifty years ago, electropolishing, chemical polishing, and replication methods were the principal specimen preparation me- ods.
Additional text
From the reviews:
“This textbook is the combined result … of four French microscopists and one Swiss microscopist who use similar TEM techniques for the study of quite different specimens; this explains the very wide scope in terms of techniques covered which distinguishes this book from related previous ones. … this recommendable book is useful for researchers and technicians working in TEM preparation laboratories. … a truly indispensable manual that is guaranteed to make it onto the shelf of almost every laboratory concerned with TEM sample preparation.” (Thomas Walther, Infocus Magazine, Issue 23, September, 2011)
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From the reviews:
"This textbook is the combined result ... of four French microscopists and one Swiss microscopist who use similar TEM techniques for the study of quite different specimens; this explains the very wide scope in terms of techniques covered which distinguishes this book from related previous ones. ... this recommendable book is useful for researchers and technicians working in TEM preparation laboratories. ... a truly indispensable manual that is guaranteed to make it onto the shelf of almost every laboratory concerned with TEM sample preparation." (Thomas Walther, Infocus Magazine, Issue 23, September, 2011)
