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Thin-layer chromatography (TLC) is widely used particularly for pharmaceutical and food analysis. While there are a number of books on the qualitative identification of chemical substances by TLC, the unique focus here is on quantitative analysis. The authors describe all steps of the analytical procedure, beginning with the basics and equipment for quantitative TLC followed by sample pretreatment and sample application, development and staining, scanning, and finally statistical and chemometric data evaluation and validation. An important feature is the coverage of effect-directed biological detection methods. Chapters are organized in a modular fashion facilitating the easy location of information about individual procedural steps.
List of contents
History of Planar Chromatography.- The Theoretical Basis of Thin-Layer Chromatography (TLC).- The Stationary Phase in Thin Layer Chromatography (TLC).- The Mobile Phase in Adsorption and Partition Chromatography.- Preparing and Applying Samples.- Basis for TLC Development Techniques.- Specific Staining Reactions.- Bioeffective-linked Analysis in Modern HPTLC.- Planar Chromatography Detectors.- Diffuse Reflectance from TLC Layers.- Fluorescence in TLC Layers.- Chemometrics in HPTLC.- Reciprocal Model.- Statistics for Quantitative TLC.- Planning an Analysis and Validation in TLC.
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From the reviews:
“An up-to-date book that reminds chromatographers and analytical chemists of the quantitative possibilities of the techniques is therefore to be welcomed. … this is a readable, well-produced and nicely illustrated volume … and a moderately useful index of approximately 14 pages. … highly recommended to anyone wishing to reacquaint themselves with a once-familiar technique that they have lost touch with. … to get up to date with the potential offered by twenty-first century instrumental TLC, this is book provides a good place to start.” (Ian D. Wilson, Chromatographia, Vol. 73, 2011)
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From the reviews:
"An up-to-date book that reminds chromatographers and analytical chemists of the quantitative possibilities of the techniques is therefore to be welcomed. ... this is a readable, well-produced and nicely illustrated volume ... and a moderately useful index of approximately 14 pages. ... highly recommended to anyone wishing to reacquaint themselves with a once-familiar technique that they have lost touch with. ... to get up to date with the potential offered by twenty-first century instrumental TLC, this is book provides a good place to start." (Ian D. Wilson, Chromatographia, Vol. 73, 2011)
