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This book gives a comprehensive account of the practical aspects of Real time PCR and its application to veterinary diagnostic laboratories. The optimisation of assays to help diagnose livestock diseases is stressed and exemplified through assembling standard operating procedures from many laboratory sources. Theoretical aspects of PCR are dealt with as well as quality control features necessary to maintain an assured testing system. The book will be helpful to all scientists involved in diagnostic applications of molecular techniques, but is designed primarily to offer developing country scientists a collection of working methods in a single source. The book is an adjunct to the Molecular Diagnostic PCR Handbook published in 2005.
List of contents
Background.- Traditional PCR.- Real-Time PCR #x2013; The Basic Principles.- New Trends in the Diagnosis and Molecular Epidemiology of Viral Diseases.- Disease Diagnosis Using Real-Time PCR Specific Procedures for Important Veterinary Pathogens.- PCR Laboratory Set-up.- Analysis and Troubleshooting.- Specifications for PCR Machines.
Summary
This book gives a comprehensive account of the practical aspects of Real time PCR and its application to veterinary diagnostic laboratories. The optimisation of assays to help diagnose livestock diseases is stressed and exemplified through assembling standard operating procedures from many laboratory sources. Theoretical aspects of PCR are dealt with as well as quality control features necessary to maintain an assured testing system. The book will be helpful to all scientists involved in diagnostic applications of molecular techniques, but is designed primarily to offer developing country scientists a collection of working methods in a single source. The book is an adjunct to the Molecular Diagnostic PCR Handbook published in 2005.
Additional text
From the reviews:
“The book Early, Rapid and Sensitive Veterinary Molecular Diagnostics–Real Time PCR Applications … is a real-time PCR ‘toolbox’ that constitutes an excellent source for users interested in learning about the practical aspects of this technology and its most relevant applications to the detection of animal disease pathogens. Its well-balanced mixture of brief theoretical treatments and extensive protocol descriptions make it a helpful resource for all scientists involved in veterinary diagnostic applications of molecular techniques.” (Mara Mirasoli, Analytical and Bioanalytical Chemistry, Vol. 400, 2011)
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From the reviews:
"The book Early, Rapid and Sensitive Veterinary Molecular Diagnostics-Real Time PCR Applications ... is a real-time PCR 'toolbox' that constitutes an excellent source for users interested in learning about the practical aspects of this technology and its most relevant applications to the detection of animal disease pathogens. Its well-balanced mixture of brief theoretical treatments and extensive protocol descriptions make it a helpful resource for all scientists involved in veterinary diagnostic applications of molecular techniques." (Mara Mirasoli, Analytical and Bioanalytical Chemistry, Vol. 400, 2011)
