Methods in Gene Technology, Volume 2

Zusammenfassung This is the second volume in a series which combines a selection of newer gene probe procedures with a review of the most important established methods! together with some examples of the ways in which gene probes can be applied.

List of contents

Contents. List of Contributors. Introduction. Purification of DNA (J.W. Dale). Electrophoretic Separation of Nucleic Acids (P.G. Sanders0. Preparative Gel Electrophoresis (J.C. Maule). Plasmid Isolation Procedures (A.I. Knight). Plasmid Isolation: column Methods (T.J. Hellyer0. Isolation of Large Plasmids (G.L. Jones and P.A. Williams). Isolation of Linear and Single-Strand Plasmid DNA (J.C. Alonso). Preparation of Bacteriophage &lgr; DNA (E. Norman). Purification of Viral RNA (J.E. Whitby, A.D.T. Barrett, A.D. Jennings, and P. Johnstone). Large Scale DNA Sequencing by Manual Methods (A.J. Davison and E.A.R. Telford). Pulsed-Field Gel Electrophoresis: Applications to Bacteria (P.R. Stewart, W. El-Adhami, and B. Inglis). Pulsed-Field Gel Electrophoresis: Large Scale Restriction Maps (N. Okahashi and T. Koga). Pulsed-Field Gel Electrophoresis: Isolation and Analysis of Large Linear Plasmids (H. Kinashi). Detection of Mutations by PCR-Single-Strand Conformation Polymorphism (A. Telenti). Analysis of DNA Supercoiling (K. Drlica, M. Malik, J.Y. Wang, A. Sasiak and R.R. Sinden). Gel Retardation Assays and DNA Footprinting (I.S. Goping, D.A. Underhill, and G.C. Shore). Southwestern Blot (G. Corte and M.T. Corsetti). Genomic Fingerprinting Using the PCR-Random Amplified Polymorphic DNA Technique (S.V.P.S. Silva and J.D. Procunier). PCR Walking (M.G. Burdon and J.J. Willoughby). Index.

Product details

• Authors: J.W. (Molecular Microbiology Group Dale, Jeremy W. Sanders Dale
• Assisted by: J.W. Dale (Editor), J.W. (Molecular Microbiology Group Dale (Editor), Jeremy W. Dale (Editor), Dale J.W. (Editor), P.G. Sanders (Editor), P.G. (Molecular Microbiology Group Sanders (Editor), Peter G. Sanders (Editor)
• Publisher: ELSEVIER SCIENCE BV
• Languages: English
• Product format: Hardback
• Released: 01.03.1995
• EAN: 9781559382649
• ISBN: 978-1-55938-264-9
• No. of pages: 370
• Subjects: Natural sciences, medicine, IT, technology > Biology > Genetics, genetic engineering; SCIENCE / Biotechnology, SCIENCE / Life Sciences / Molecular Biology, SCIENCE / Life Sciences / Genetics & Genomics, biotechnology, Genetics (non-medical)