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The standardization of meristem culture for banana virus eradication from infected plant (Amritasagar and Sabri) is now an efficient practice for rapid clonal propagation. Generally, four experiments were conducted for shoot proliferation & virus free plantlet regeneration confirmed by ELISA test could play an important role. Several factors like explants, media composition, environment, ELISA test, PGR,s concentrations & combinations were included that. Usually, cytokinin helps in shoot proliferation & auxins on in vitro root formation. However,cytokinin & auxins requirement depends on variety & culture conditions. Moreover,an exponential shoot number increment & very short(2-6weeks)shoot proliferation cycle can be continued irrespective of the season. But conventionally, no uniform & no virus free 5-10 suckers can be obtained per plant annually. As regards yield performance,tissue cultured plants have been reported to produce 39%higher yield than sword suckers. Finally, virus eradication by meristem culture is a useful tactic that could be used as an effective tool for many experiments for commercial exploitation of virus free banana plant production in different level in future.
About the author
Md.Humayun Kabir Shiragi,born 1975 & Bangladeshi,has obtained his MS in Horticulture,2004 & B Sc Ag.from Bangladesh Agricultural University. Since then,he has worked in different orgs.In 2007,he has joined Soil Resource Development Institute as scientific officer.He is the author of several articles published in reputed journals & member two orgs.
