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This volume provides a variety of standard protocols used to cryopreserve or freeze-dry different types of specimens. In addition, it provides chapters focused on the fundamental principles of cryopreservation, vitrification, and freeze-drying. Several state of the art microscopic, spectroscopic as well as calorimetric methods are highlighted that can be used to study cellular and macromolecular changes in response to freezing or drying. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Cryopreservation and Freeze-Drying Protocols, Third Edition serves as a practical guideline for studies on freezing and drying processes as well as preservation strategies for biological specimens.
List of contents
Principles of Cryopreservation.- Principles of Cryopreservation By Vitrification.- Modeling and Optimization of Cryopreservation.- The Principles of Freeze-Drying.- Use of In Situ Fourier Transform Infrared Spectroscopy to Study Freezingand Drying of Cells.- Calorimetric Analysis Of Cryopreservation and Freeze-Drying Formulations.- Measurement Of Intracellular Ice Formation Kinetics By High-Speed Video Cryomicroscopy.- Laser Scanning Microscopy in Cryobiology.- Low Temperature Electron Microscopy.- Cryopreservation of Semen From Domestic Livestock.- Cryopreservation of Mammalian Oocytes.- Vitrification: A Simple And Successful Method for Cryostorage of Human Blastocysts.- Efficient Cryopreservation of Human Pluripotent Stem Cells By Surface-Based Vitrification.- Cryopreservation Of Greenshell(TM) Mussel (Perna Canaliculus) Sperm.- Membrane Modification Strategies for Cryopreservation.- Sperm Clean-Up and Density Centrifugation Procedures.- Cryopreservation of Red Blood Cells.- Cord Blood Clinical Processing, Cryopreservation, and Storage.- Directional Freezing For Large Volume Cryopreservation.- Vitrification Of Heart Valve Tissues.- Cryopreservation of Plant Cell Lines.- Writing Standard Operating Procedures (Sops) For Cryostorage Protocols: Using Shoot Meristem Cryopreservation As An Example.- Freeze-Drying Of Proteins.- Freeze-Drying Of Lactic Acid Bacteria.- Freeze-Drying Of Mammalian Sperm.- Freeze-Drying Of Decellularized Heart Valve Tissues.
Summary
This volume provides a variety of standard protocols used to cryopreserve or freeze-dry different types of specimens. In addition, it provides chapters focused on the fundamental principles of cryopreservation, vitrification, and freeze-drying. Several state of the art microscopic, spectroscopic as well as calorimetric methods are highlighted that can be used to study cellular and macromolecular changes in response to freezing or drying. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Cryopreservation and Freeze-Drying Protocols, Third Edition serves as a practical guideline for studies on freezing and drying processes as well as preservation strategies for biological specimens.
