A Laboratory Guide to Glycoconjugate Analysis

18. 2 Principle of FACE/Gel Retardation Assay . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 349 18. 3 Labelling of Oligosaccharides with ANTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 350 18. 4 Screening of Carbohydrate Ligands for Proteins . . . . . . . . . . . . . . . . . . . . . . . . . . . 352 18. 5 Measurement of Binding Constant for the Interaction Between Protein and ANTS-Labelled Carbohydrate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 355 18. 6 Measurement of Binding Constant for the Interaction Between Protein and Native Carbohydrate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 357 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 360 ~ The Application of Capillary Affinity Electrophoresis to the Analysis _ of Carbohydrate-Protein Interactions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 361 19. 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 361 19. 2 Principle of CAE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 363 19. 3 Determination of Association Constants . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 364 19. 4 Technical Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 366 General considerations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 366 19. 5 Limitations of the Technique . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . . . . . . . . . 370 19. 6 Application of CAE to the Analysis of Carbohydrate-Protein Interactions . . . . . . 371 19. 7 Conclusions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 375 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 377 20. 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 379 Definitions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 380 20. 2 Technical Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 381 20. 3 Sample Detection and Sample Recovery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 389 Autoradiography and staining . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 389 Sample detection by blotting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 389 Semipreparative ACE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 390 20. 4 Analysis of Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 391 Measuring sample mobilities - calculating a retardation coefficient . . . . . . . . . . . . 391 Graphical analysis of data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 392 Interpreting ACE patterns . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 393 Reverse ACE . . . .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 395 20. 5 Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 397 Acknowledgements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 398 References . . . . . . .

List of contents

1 High-Performance Liquid Chromatography of Derivatized and Non-Derivatized Oligosaccharides: A Review.- 2 Detection of the Presence of Carbohydrates in Glycoproteins.- 3 HPAE-PAD Monosaccharide and Oligosaccharide Analysis of Glycoproteins Electrotransferred onto Polyvinylidene Fluoride Membranes.- 4 Determination of the Disaccharide Composition of Glycosaminoglycans: Comparison of Chemical and Enzymatic Scission.- 5 Mass Spectrometric Analysis of Highly Acidic Polysaccharides.- 6 Analysis of N-Glycans by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry.- 7 Polyacrylamide Gel Electrophoresis of Fluorophore-Labelled Reducing Saccharides: A Review.- 8 Carbohydrate Analysis with Capillary Electrophoresis.- 9 Oligosaccharide Profiling of Keratan Sulphate.- 10 Analysis of the Structure of Heparin and Heparan Sulfate by High-Resolution Separation of Oligosaccharides.- 11 HPLC Strategies for Profiling and Sequencing Oligosaccharides.- 12 Enzymatic Sequence Analysis of Glycoprotein Glycans.- 13 Exosequencing of Heparan Sulphate/Heparin Saccharides Using Lysosomal Enzymes.- 14 The Application of Two-Dimensional Capillary Electrophoresis to the Identification of Glycan Structures.- 15 The Application of Three-Dimensional HPLC to the Identification of N-linked Oligosaccharide Structures.- 16 Simultaneous Fluorescent Labelling and Biotinylation of Oligosaccharides: A Versatile Approach to the Analysis of Oligosaccharide Structure and Function.- 17 Preparation of Neoglycolipids for Structure and Function Assignments of Oligosaccharides.- 18 Analysis of Protein - Carbohydrate Interactions by FACE/Gel Retardation Assay.- 19 The Application of Capillary Affinity Electrophoresis to the Analysis of Carbohydrate-Protein Interactions.- 20 Analysis ofProtein-Glycosaminoglycan Interactions by Affinity Co-Electrophoresis.

Product details

• Assisted by: J. T. Gallagher (Editor), J.T. Gallagher (Editor), Jackson (Editor), P Jackson (Editor), P. Jackson (Editor), T Gallagher (Editor), T Gallagher (Editor)
• Publisher: Springer, Basel
• Languages: English
• Product format: Paperback / Softback
• Released: 01.01.2012
• EAN: 9783034873901
• ISBN: 978-3-0-3487390-1
• No. of pages: 410
• Dimensions: 170 mm x 23 mm x 244 mm
• Weight: 748 g
• Illustrations: XXII, 410 p. 724 illus.
• Series: Biomethods; Biomethods
• Subject: Natural sciences, medicine, IT, technology > Biology > Biochemistry, biophysics