Protein Phosphorylation - A Practical Approach

Klappentext Reversible phosphorylation is one of the major mechanisms of controlling protein activity in all eukaryotic cells. This new edition of Protein Phosphorylation: A Practical Approach provides a comprehensive description of current methods used to study protein phosphorylation and the kinases and phosphatases which catalyse it. It includes protocols for studying phosphorylation in intact cells; analysis of signal transduction pathways! kinase specificity! and kinase interactions; assay and purification of kinases and phosphatases; and identification of substrates. Also covered are cloning and expression protocols and advice on the crystallization of kinases and phosphatases. Protein Phosphorylation: A Practical Approach 2e will therefore be of great value to any researcher investigating aspects of reversible protein phosphorylation. Zusammenfassung Reversible protein phosphorlyation is one of the major mechanisms of controlling protein activity. This new edition provides a comprehensive description of current methods used to investigate this important phenomenon. Preface; 1. Study of protein phosphorylation in intact cells; 2. Analysis of signal transduction pathways using protein kinase inhibitors and activators; 3. Analysis of signal transduction pathways using proteinserine/threonine phosphatase inhibitors; 4. Analysis of signal transduction pathways using molecular biological manipulations; 5. Phosphopeptide mapping and phosphoamino acid analysis on cellulose thin-layer plates; 6. Phosphorylation site analysis by mass spectrometry; 7. Assay and purification of protein (serine/threonine) phosphatases; 8. [chapter title missing]; 9. Identification of substrates for protein-tyrosine phosphatases; 10. Assay and purification of protein-serine/threonine kinases; 11. Assay of protein kinases and phosphatases using specific peptide substrates; 12. Cloning and expression of cDNAs encoding protein kinase subunits; 13. Interaction cloning of protein kinase partners; 14. Identifying protein kinase substrates by expression screening with solid-phase phosphorylation; 15. Analysis of protein kinase interactions using the two-hybrid method; 16. Analysis of protein kinase interactions using biomolecular interaction analysis; 17. Analysis of protein kinase specificity using orientated peptide libraries; 18. Crystallization of protein kinases and phosphatases; Index...

Product details

• Authors: D. Grahame Hardie, Grahame Hardie
• Assisted by: D. G. Hardie (Editor), Grahame Hardie (Editor), Grahame (Department of Biochemistry Hardie (Editor)
• Publisher: Oxford University Press
• Languages: English
• Product format: Paperback / Softback
• Released: 14.10.1999
• EAN: 9780199637287
• ISBN: 978-0-19-963728-7
• No. of pages: 460
• Series: Practical Approach Series; Practical Approach (Paperback); Practical Approach (Paperback)
• Subject: Natural sciences, medicine, IT, technology > Biology > Genetics, genetic engineering