Gel Electrophoresis of Proteins - A Practical Approach

Klappentext This new edition is almost a completely new text! with eight of the ten chapters written by new authors. It presents the most reliable methods for essential procedures such as one-dimensional polyacrylamide gel electrophoresis! isoelectric focusing! two-dimensional gel electrophoresis! preparative gel electrophoresis! and peptide mapping! complete with the latest refinements of the procedures. In addition! it describes major new techniques developed since the previous edition. These include capillary gel electrophoresis! sequence analysis of gel-resolved proteins! fluorophore-labelled saccharide electrophoresis! and analysis of protein-protein interactions by gel electrophoresis. Like the previous editions! this volume is easy-to-follow and thorough! a laboratory manual written by experienced researchers for researchers. The emphasis is on describing the bestmethods! in step-by-step detail! with copious advice to ensure that each method works the first time. This new edition is a 'must' for anyone currently uses gel electrophoresis. Zusammenfassung This text presents the best methods, hints and tips for core procedures such as one-dimensional polyacrylamide gel electrophoresis, isoelectric focusing, two-dimensional gel electrophoresis, preparative gel electrophoresis, and peptide mapping, with refinements and updates of the procedures. Inhaltsverzeichnis 1: One-dimensional PAGE 2: Protein detection 3: Preparative gel electrophoresis 4: Capillary SDS electrophoresis 5: Conventional isoelectric focusing 6: 2-D gel electrophoresis 7: Peptide mapping 8: Sequence analysis of gel-resolved proteins 9: Analysis of glycoproteins 10: Protein: protein interactions Appendix 1 - List of suppliers Appendix 2 - Bibliography of polypeptide detection methods

Product details

• Authors: Hames, B. D. (Department of Biochemistry and Molec Hames, B. D. Rickwood Hames, B.d. Rickwood Hames
• Assisted by: B. D. Hames (Editor), B. David Hames (Editor), Hames B. D. (Editor), D. Rickwood (Editor)
• Publisher: Oxford University Press
• Languages: English
• Product format: Paperback / Softback
• Released: 01.10.1998
• EAN: 9780199636402
• ISBN: 978-0-19-963640-2
• No. of pages: 372
• Series: Practical Approach Series; Practical Approach Series
• Subjects: Natural sciences, medicine, IT, technology > Biology > Biochemistry, biophysics; SCIENCE / Life Sciences / Biology, SCIENCE / Life Sciences / Biochemistry, SCIENCE / Chemistry / Organic, biochemistry, proteins, Polymer chemistry, Chemical biology