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Zusatztext This book is very useful for practising antibody engineers, providing extra information that enables them to adopt new techniques or expand into new areas. ... this is a useful and interesting resource for practising antibody engineers and I will make sure my students have access to it. Klappentext Recombinant DNA techniques have revolutionized the isolation and production of antibodies in recent years. This has resulted in rapid changes in how to handle antibodies for research. This new Practical Approach volume responds to this change by assembling! in one volume! protocols which allow the researcher to isolate a new antibody! analyse its properties! format the right antibody molecule or fragment! and produce it in usable quantities. The book is divided into two parts: the first describes the generation and analysis of antibodies and the second covers engineering and production. This timely book will be of interest to those involved in both clinical and molecular biological applications of antibodies. Zusammenfassung Recombinant DNA techniques have revolutionized the isolation and production of antibodies in recent years, and rapid changes have been made. This new Practical Approach book details protocols which allow the researcher to isolate a new antibody, analyse its properties, format the right antibody molecule or fragment, and produce it in usable quantities. Inhaltsverzeichnis 1: Construction and use of antibody gene repertoires 2: Affinity maturation of antibodies using phage display 3: Human antibody repertoires in transgenic mice: manupulation and transfer of YACs 4: Measuring antibody affinities in solution 5: Measuring antibody affinities using Bio-sensors 6: Analysis of human antibody sequences 7: Rodent to human antibodies by CDR grafting 8: Rodent to human antibodies by phage display 9: Choosing and manipulating effector functions 10: Secretion and purification of antibody fragments expressed in bacteria 11: Production of single chain Fv monomers and multimers 12: Expression of immunoglobulin genes in mammalian cells 13: In vitro linkage of antibody fragments into dimeric and multimeric forms ...
