Neural Cell Culture - A Practical Approach

Zusatztext This practical book describes tried and tested methods for preparing cultures from a diverse range of tissues and cells from the central and peripheral nervous systems of mammals and birds. Klappentext The immense complexity and cellular heterogeneity of the vertebrate nervous system makes it difficult to study many types of neural processes at a cellular and molecular level. In recent years! the establishment of cell culture model systems for almost every region and cell type in the nervous system has been crucial in facilitating such studies and in heralding some key breakthroughs in our understanding of the underlying mechanisms.Neural Cell Culture brings together methods for preparing cultures from a diverse range of neural tissues and cells! from both the central and peripheral nervous systems of mammalian and avian species. Important features of this volume are the clearly illustrated dissection guides accompanying each chapter! methods for enriching and purifying distinct cell types! and guidance on optimizing cell viability and growth. Each chapter includes valuable step-by-step protocols! hints and tips for success! advice! and background information. Zusammenfassung This book brings together methods for preparing and maintaining cell cultures from a diverse range of neural tissues and cells! from both the central and peripheral nervous systems. Inhaltsverzeichnis Part I - CNS Neurons 1: Cerebellum: granule cells 2: Cerebellar Purkinje neurons 3: Nigral and straital neurons 4: Septal neurons 5: Proliferation and differentiation in retinal cell cultures 6: Purified embryonic motoneurones Part II - CNS glia and non-neuronal cells 7: Astrocytes 8: Oligodendrocyte lineage cells from neonatal rat brain 9: Micrglia: the tissue macrophage of the CNS 10: Brain endothelium Part III - PNS neurons and glia 11: Neural crest cells: strategies to generate lineage diversifaction in vitro 13: Avian cilliary and lumbar sympathetic ganglion neurons 14: Enteric ganglion cells 15: Mouse olfactory epithelium 16: Schwann cell culture ...

Product details

• Authors: James (Reader in Cellular Neurobiology Cohen, James Ed Cohen, James Ed. Cohen, James Wilkin Cohen, Wilkin Cohen, Cohen Wilkin
• Assisted by: Cohen (Editor), James Cohen (Editor), Graham Wilkin (Editor), Graham P. Wilkin (Editor), Wilkin Graham P. (Editor)
• Publisher: Oxford University Press
• Languages: English
• Product format: Paperback / Softback
• Released: 14.03.1996
• EAN: 9780199634842
• ISBN: 978-0-19-963484-2
• No. of pages: 268
• Series: Practical Approach Series; Practical Approach Series
• Subjects: Natural sciences, medicine, IT, technology > Medicine > Non-clinical medicine; SCIENCE / Life Sciences / Zoology / General, SCIENCE / Life Sciences / Neuroscience, SCIENCE / Life Sciences / Cell Biology, Zoology & animal sciences, Neurosciences, Cellular biology (cytology), Zoology and animal sciences