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Zusatztext The present book consists of 7 chapters that each coprehensively describe different aspects of the subject and which may be read independently ... this book is very detailed and informative and contains much information with key literature citations of use for any scientist working in the area of heterologous protein expression ... The book can thus be highly recommended. Klappentext Expression systems are in widespread use for cloning specific genes! for synthesizing the encoded proteins for structural and functional analysis! and for large-scale preparation of current use with background information and advice on the merits of each! step-by-step practical protocols! troubleshooting! and details of the latest applications. These two books! the first of four volumes are thoroughly revised and up-dated versions of the original ones published in the Practical Approach series. Together they will offer a complete guide to the major techniques required by modern molecular biology laboratories. Zusammenfassung Expression systems are in widespread use for cloning specific genes, for synthesising the encoded proteins for structural and functional analysis, and for large-scale preparation of current use with background information and advice on the merits of each, step-by-step practical protocols, troubleshooting, and details of the latest applications. These two books, the first of four volumes are thoroughly revised and up-dated versions of the original ones published in the Practical Approach series. Together they will offer a complete guide to the major techniques required by modern molecularbiology laboratories. Inhaltsverzeichnis 1.: Screening expression libraries with antibody and protein probes 2.: Expression of foreign proteins in E.coli using plasmid vectors and purification of specific polyclonal antibodies 3.: Purification of over-produced proteins from E.coli cells 4.: Production of monoclonal antibodies against proteins expressed in E.coli 5.: Expression of cloned genes in yeast 6.: Intraction trap cloning with yeast 7.: The baculovirus expression system ...
