Dna Cloning 1: A Practical Approach - Core Techniques

Klappentext This volume provides detailed laboratory protocols! advice! hints and tips! example data! key literature citations! background information! and troubleshooting comments for the steps used in most molecular biology laboratories for example transformation! construction of cDNA and genomic libraries! probe preparation! analysis of gene organization and expression! in vitro mutagenesis! and DNA sequencing. The information provided is an completely up-to-date account of each topic by established researchers. These two books! the first of four volumes are thoroughly revised and up-dated versions of the original ones published in the Practical Approach series. Together they will offer a complete guide to the major techniques required by modern molecular biology laboratories. Zusammenfassung This volume provides detailed laboratory protocols, advice, hints and tips, example data, key literature citations, background information, and troubleshooting comments for the steps used in most molecular biology laboratories. It covers transformation, construction of cDNA and genomic libraries, probe preparation, analysis of gene organisation and expression, in vitro mutagenesis, and DNA sequencing. The information provided is a completely up to date account of each topic by established researchers. These two volumes, the first of four, are a thoroughly revised and up dated version of the original ones published in the Practial Approach series. Together they will offer a complete guide to the major techniques required by modern molecular biology laboratories. Inhaltsverzeichnis 1.: Techniques for transformation of E.coli 2.: Lambda replacement vectors in the construction of genomic DNA libraries 3.: Procedures for cDNA cloning 4.: Making probes 5.: Using cloned DNAs to analyse gene organization and expression 6.: Choices of single-stranded vectors and their application for in vitro mutagenesis 7.: PCR and its applications 8.: DNA sequencing ...

Product details

• Authors: D. M. Glover, D. M. (Professor Glover, D. M. Hames Glover, David M. Glover, David M. Hames Glover, Hames Glover, Glover Hames, David Hames
• Assisted by: D. M. Glover (Editor), Glover D. M. (Editor), B. D. Hames (Editor), Hames B. D. (Editor)
• Publisher: Oxford University Press
• Languages: English
• Product format: Paperback / Softback
• Released: 11.05.1995
• EAN: 9780199634767
• ISBN: 978-0-19-963476-7
• No. of pages: 288
• Series: Practical Approach Series; Practical Approach Series
• Subjects: Humanities, art, music > Linguistics and literary studies > General and comparative linguistics; Natural sciences, medicine, IT, technology > Biology > General, dictionaries; Medical research, SCIENCE / Life Sciences / Biochemistry, SCIENCE / Life Sciences / Molecular Biology, SCIENCE / Life Sciences / Genetics & Genomics, MEDICAL / Research, biochemistry, molecular biology, Genetics (non-medical)