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Zusatztext Growth areas in neurochemistry in the ten years since the first edition was published are reflected in this fully updated second edition. Klappentext Neurobiology has undergone a revolution with many advances dependent on new techniques and approaches. This fully updated second edition reflects the recent growth areas in this subject. Its major themes are the fractionation and study of cellular and subcellular components of the nervous system! chemical neuroanatomy! and the measurement of neuronal metabolism in vivo and in vitro. Topics include the neuronal cytoskeleton; isolation of synaptosomes and their subcellular components; aspects of mitochondrial defects in neurones; and synaptic transport processes exemplified by the GABA transporter. This is an invaluable practical laboratory manual for all researchers in neurobiology; it contains step-by-step protocols and a troubleshooting guide! as well as examples of experimental data and their interpretation. Zusammenfassung Neurochemistry is the study of the cellular and subcellular components of the nervous system and the molecules found within it. The nervous system is complex and neurochemistry a diverse field in which there has been much interest recently, for example in the increased awareness of pathological brain conditions such as Alzheimer's disease, depression, and mania. A wide range of techniques are avialable to the neurochemist and this volume contains the core elements of neurochemistry that will be useful to both first time researchers and established investigators in all fields of neuroscience. Inhaltsverzeichnis 1.: Isolation of synaptosomes, growth cones and their subcellular components 2.: Proteins of the neuronal cytoplasmic and membrane-associated cytoskeleton 3.: Immunocytochemical techniques for the study of the nervous system 4.: In situ hybridization approaches to the study of the nervous sytem 5.: Investigation of mitochondrial defects in brain and skeletal muscle 6.: Measurement of neurotransmitters and their associated second messengers in vivo 7.: Protein phosphorylation and dephosphorylation in the nervous system 8.: Receptor-linked phosphoinositide metabolism 9.: Neurotransmitter transporters: purification, reconstitution, and assay of the brain GABA transporter ...
