In Situ Hybridization - Principles and Practice

Zusatztext Anyone contemplating using in situ hybridisation in their laboratory will need a thorough grounding in the background and principles of the methods. This book excels in its background presentation, with clear explanations and good referencing.....There are very fewbooks dealing with the practicalities of in situ hybridisation. This one makes a major contribution to the subject and therefore is invaluable as a reference for many scientists and clinicians Dr Deborah K Dun-Waters, The Bulletin of the Royal College of Pathologists, No. 107 June 1999 Klappentext This practical guide covers all aspects of in situ hybridization, describing: practical procedures and protocols, the scientific background, areas of application, and the limitations of the technology. This new edition has been completely rewritten by the experts in the field to take into account the many advances in the last seven years. Zusammenfassung The technique of in situ hybridisation is vital to molecular biologists and their understanding of the pathophysiology of cellular functions. This practical guide covers all aspects of in situ hybridisation, describing: practical procedures and protocols, the scientific background, areas of application, and the limitations of the technology. Inhaltsverzeichnis 1: Principles of in situ hybridization 2: The design and generation of probes for in situ hybridization 3: The preparation of non-radioisotopic hybridization probes 4: Principles and applications of complementary RNA probes 5: Quantification of in situ hybridization 6: Quantification of grains in in situ hybridization 7: Detection of genetic changes in cancer by interphase cytogenetics and comparative genomic hybridization 8: Single and simultaneous nucleic acid detection in clinical samples 9: Combined in situ hybridization and immunocytochemistry: investigation of viral pathogenesis at the light and electron microscope levels 10: Super sensitive in situ DNA and RNA localisation ...

Product details

• Authors: &apos, Mushir d. Hasan, Polak, Julia M. ( Polak, Julia M. (Department of Histochemistry Polak, Julia M. Mcgee Polak
• Assisted by: James Mcgee (Editor), James O'D. McGee (Editor), McGee James O'D. (Editor), Julia Polak (Editor), Julia M. Polak (Editor), Polak Julia M. (Editor)
• Publisher: Oxford University Press
• Languages: English
• Product format: Hardback
• Released: 28.01.1999
• EAN: 9780198548805
• ISBN: 978-0-19-854880-5
• No. of pages: 224
• Series: Oxford Medical Publications
• Subjects: Natural sciences, medicine, IT, technology > Medicine > Clinical medicine; Microbiology (non-medical), SCIENCE / Life Sciences / Developmental Biology, SCIENCE / Life Sciences / Molecular Biology, SCIENCE / Life Sciences / Cell Biology, SCIENCE / Life Sciences / Microbiology, MEDICAL / Laboratory Medicine, molecular biology, Cellular biology (cytology), Developmental biology, Medical laboratory testing & techniques, Medical laboratory testing and techniques