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Informationen zum Autor Dr Tim ClacksonVice President, Gene Therapy and GenomicsARIAD Pharmaceuticals, Inc., 26 Landsdowne Street, Cambridge, MA 02139, USA+1 617 494 0400 ext. 258+1 617 225 2589clackson@ariad.comTim Clackson, Ph.D. is Senior Vice President, Science and Technology at ARIAD Pharmaceuticals, Inc., where he has served for eight years in a variety of research and research management positions. His group is combining chemistry, cell biology and protein engineering to develop techniques for regulating gene expression and other intracellular processes using small molecules. Dr Clackson's particular research interest is the use of protein engineering and chemical biology techniques to analyze and redesign the interfaces between molecules, especially small molecule-protein interfaces.Dr Henry B. LowmanSenior ScientistGenentech Inc., 1 DNA Way, South San Francisco, CA 94080, USA+1 650 225 1171+1 650 225 3734hbl@gene.comHenry B. Lowman, Ph.D. is a Senior Scientist and Associate Director of Antibody Engineering with joint appointments in Immunology and Protein Engineering, at Genentech, Inc., where he has been engaged in research on proteins and peptides for over thirteen years. Klappentext Phage display has become established as a powerful protein engineering method for identifying polypeptides with novel properties! and altering the properties of existing ones. Although the technique is widely used in biological research and drug discovery! it remains technically challenging! and new applications and procedures continue to evolve. Phage Display--A Practical Approach is an up-to-date! comprehensive and integrated experimental guide to the technique! useful for novice and expert alike. Zusammenfassung Designed to enable researchers to design and undertake a phage display project, from designing an experimental strategy and constructing a library to performing selections and analyzing the results. The protocols and chapters in this book are cross-referenced, allowing readers to move beyond the specific examples provided. 1. Introduction to phage biology and phage display; 2. Constructing phage display libraries by oligonucleotide-directed mutagenesis; 3. In vitro DNA recombination; 4. Phage selection strategies for improved affinity and specificity of proteins and peptides; 5. Rapid screening of phage displayed protein binding affinities by phage ELISA; 6. Identification of novel ligands for receptors using recombinant peptide libraries; 7. Substrate phage display; 8. Protease-based selection of stably folded proteins and protein domains from phage display libraries; 9. Phage display of zinc fingers and other nucleic acid-binding motifs; 10. In vivo and ex vivo selections using phage-displayed libraries; 11. Screening phage libraries with sera; 12. Interaction cloning using cDNA libraries displayed on phage; 13. Phage antibody libraries; 14. Affinity maturation of phage antibodies...
