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Informationen zum Autor Muhammad Sohail Klappentext Describes methods and protocols for gene silencing and RNA interference. From the design and production of RNAi tools to their applications! this book presents the spectrum of activity! design! production! and delivery of RNAi reagents. Zusammenfassung Describes methods and protocols for gene silencing and RNA interference. From the design and production of RNAi tools to their applications, this book presents the spectrum of activity, design, production, and delivery of RNAi reagents. Inhaltsverzeichnis Gene silencing by RNA interference and the role of small interfering RNAs; N.J. Caplen! National Institutes of Health! Maryland! USABasics of siRNA design and chemical synthesis; Q. Boese! A. Reynolds! and W.S. Marshall! Dharmacon! Inc. Colorado! USAOligonucleotide scanning arrays in the design of small interfering RNAs; V.M. Macaulay and M. Sohail! Weatherall Institute of Molecular Medicine and University of Oxford! England! UKsiRNA production by in vitro transcription; O. Donzé! P. Dudek! and D. Picard! Université de Genève and Apotech Corporation! SwitzerlandProduction of siRNAs with the application of deoxyribozymes; G. Doran and M. Sohail! University of Oxford! England! UKProduction of siRNA in vitro by enzymatic digestion of double-stranded RNA; F. Buchholz! D. Drechsel! M. Ruer! and R. Kittler! Max Planck Institute! Dresden! GermanyPlasmid-mediated intracellular expression of siRNAs; R. Wadhwa! M. Sano! M. Miyagishi! and K. Taira! National Institute of Advanced Industrial Science and Technology! Tsukuba! and University of Tokyo! JapanLentiviral vector-mediated delivery of si/shRNA! D.S. An! X-F. Qin! Vincent Auyeung! D. Baltimore! and I.S.Y. Chen! University of California-Los Angeles! UT MD Anderson Cancer Center in Texas! and California Institute of Technology! Pasadena! USAExogenous siRNA delivery: protocols for optimizing delivery to cells; A.J. Hollins! S.P. Fox! and S. Akhtar! Cardiff University! Wales! UKRNAi in Drosophila cell cultures! M. Bettencourt-Dias! R. Sinka! L. Frenz! and D. Glover! University of Cambridge. and Cyclacel! Ltd.! England! UKRNAi in Caenorhabditis elegans; F. Simmer and R.H.A. Plasterk! Center for Biomedical Genetics! Utrecht! The NetherlandsDelivery of RNAi reagents in C. elegans by microinjection; P. Appleford and A. Woollard! University of Oxford! England! UKRNAi in Drosophila embryos; R.W. Williams! University of California! Berkeley! USARNAi in Xenopus laevis oocytes; E. Gordon and G.W. Abbott! Cornell University! New York! USARNAi in Xenopus embryos; K.H. Kok and D-Y. Jin! University of Hong Kong! People's Republic of ChinaAn RNAi-based genomic library for forward genetics in the African Trypanosome; J.C. Morris! Z. Wang! S.A. Motyka! M.E. Drew! and P.T. Englund! Clemson University! South Carolina! Massachusetts Institute of Technology! Massachusetts! Johns Hopkins University! Maryland! and Washington University! Missouri! USARNAi in the malaria parasite Plasmodium; A. Brüning-Richardson and G.A. McConkey! University of Leeds! England! UKSilencing the expression of cysteine proteases (falcipains) in Plasmodium falciparum using RNA interference approaches; P.V.N. Dasaradhi! A. Mohmmed! M.J. Hossain! V.S. Chauhan! and P. Malhotra! International Center for Genetic Engineering and Biotechnology! New Delhi! IndiaRNA in transgenic animal models; H. Hasuwa & M. Okabe! Osaka University! JapanRNA interference in mouse model! E. Song! P. Stern! D. Palliser! Luk Van Parijs and J. Lieberman! Harvard University! Massachusetts! USAVirus-induced RNA silencing in plants; M. Figlerowicz! M. Barciszewska-Pacak! B. Grabowska! J. Wojciechowicz! and A. Pacak! Adam Mickiewicz University! Poznan! University of Warmia and Mazury! Olsztyn! and Polish Academy of Sciences! Poznan! PolandRNA silencing in plants - biolistic delivery of RNAi reagents; U. Klahre and F. Meins! Novartis Research Foundation! Friedrich Mie...
