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Informationen zum Autor BRYAN M. HAM, PhD, is a member of the American Society for Mass Spectrometry and the American Chemical Society. He has conducted proteomics and lipidomics research at The Ohio State University and Pacific Northwest National Laboratory in Richland, Washington. He is currently working for the Department of Homeland Security at the U.S. Customs and Border Protection New York Laboratory. He has published numerous research papers in peer-reviewed journals, and is the author of Even Electron Mass Spectrometry with Biomolecule Applications (Wiley). Klappentext Obtain protein phosphorylation knowledge using mass spectrometry techniquesA practical introduction to mass spectrometry-based phosphoproteomic studies, this book addresses various concepts including the protocols necessary for sample preparation through mass spectral interpretation of protein phosphorylation events. It delves into the determining factors for whether or not a protein has been correctly modified by the addition of a phosphate group. Highlighting both basic and significant principles, as well as specific applications using examples, Proteomics of Biological Systems covers:* Key fundamentals of mass spectrometry including mass analyzers, ionization, and nano-electrospray/nano-flow liquid chromatography* Optimized methodologies for performing post translational modification (PTM) of protein studies using tried and tested sample preparation and analysis methods and approaches* Specific examples of eukaryote and prokaryote normal state and perturbed state phosphoproteomic studies* Use of the most recent systems biology bioinformatic Internet tools for interpreting complex data such as the Blast2GO gene ontology tool* Step-by-step methodology for phosphoproteomic studies of both eukaryote and prokaryote systemsThis unique resource contains specific chapters devoted to normal state HeLa cell phosphoproteomes, DNA damage response state HeLa cell phosphoproteomes, Caulobacter crescentus normal state phosphoproteomes, and more. A core guide for scientists and researchers in academic laboratories and pharmaceutical, chemical, and biotech companies, Proteomics of Biological Systems is a useful text for expanding comprehension of mass spectrometry-based phosphoproteomic study. Zusammenfassung Phosphorylation is the addition of a phosphate (PO 4) group to a protein or other organic molecule. Phosphorylation activates or deactivates many protein enzymes, causing or preventing the mechanisms of diseases such as cancer and diabetes. Inhaltsverzeichnis Preface xvii Acknowledgments xxi About the Author xxiii 1 Posttranslational Modification (PTM) of Proteins 1 1.1 Over 200 Forms of PTM of Proteins 1 1.2 Three Main Types of PTM Studied by MS 2 1.3 Overview of Nano-Electrospray/Nanofl ow LC-MS 2 1.3.1 Defi nition and Description of MS 2 1.3.2 Basic Design of Mass Analyzer Instrumentation 3 1.3.3 ESI 7 1.3.4 Nano-ESI 11 1.4 Overview of Nucleic Acids 15 1.5 Proteins and Proteomics 20 1.5.1 Introduction to Proteomics 20 1.5.2 Protein Structure and Chemistry 22 1.5.3 Bottom-Up Proteomics: MS of Peptides 27 1.5.3.1 History and Strategy 27 1.5.3.2 Protein Identifi cation through Product Ion Spectra 30 1.5.3.3 High-Energy Product Ions 36 1.5.3.4 De Novo Sequencing 37 1.5.3.5 Electron Capture Dissociation (ECD) 40 1.5.4 Top-Down Proteomics: MS of Intact Proteins 42 1.5.4.1 Background 42 1.5.4.2 GP Basicity and Protein Charging 42 1.5.4.3 Calculation of Charge State and Molecular Weight 44 1.5.4.4 Top-Down Protein Sequencing 46 1.5.5 Systems Biology and Bioinformatics 48 1.5.6 Biomarkers in Cancer 52 Reference 56 2 Glycosylation of Proteins 59 2.1 Production of a Glyc...
