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Klappentext The critically acclaimed laboratory standard for almost 50 years! Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Each volume is eagerly awaited! frequently consulted! and praised by researchers and reviewers alike. Now with over 520 volumes and 40!000 chapters in the collection! much of the material is still relevant today and is truly an essential publication for researchers in all fields of life sciences! including microbiology! biochemistry! cancer research! and genetics! just to name a few. In this volume! number 545! we have brought together a number of core protocols concentrating on protein! carefully written and edited by experts.
List of contents
1. Purification of His-tagged ProteinsAnne Spriestersbach, Jan Kubicek, Frank Schäfer, Helena Block and Barbara Maertens2. Affinity Purification of a Recombinant Protein Expressed as a Fusion with the Maltose-Binding Protein (MBP) TagKrisna C. Duong-Ly and Sandra B. Gabelli3. Immunoaffinity Purification of ProteinsJennifer M. Kavran and Daniel J. Leahy4. Affinity Purification of Protein Complexes Using TAP TagsErica Gerace and Danesh Moazed5. Strep-tagged Protein PurificationBarbara Maertens, Anne Spriestersbach, Jan Kubicek and Frank Schäfer6. Proteolytic Affinity Tag CleavageHelena Block, Barbara Maertens, Anne Spriestersbach, Jan Kubicek and Frank Schäfer7. Affinity Pull-down of Proteins using anti-FLAG M2 Agarose BeadsErica Gerace and Danesh Moazed8. Protein Affinity Purification using Intein/Chitin Binding Protein TagsSarah F. Mitchell and Jon R. Lorsch9. Purification of GST-tagged ProteinsFrank Schäfer, Nicole Seip, Barbara Maertens, Helena Block and Jan Kubicek
