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Provides students with the solid grounding in biological laboratory techniques needed to pursue any major in the life and health sciences
Building on the success of its American Publishers Award-winning predecessor, Current Protocols Essential Laboratory Techniques, Second Edition gives new researchers the skills and understanding of the fundamental laboratory procedures that are needed to run experiments, solve problems, and become a productive member of the modern life science laboratory.
An indispensable tool for readers, this Second Edition includes chapters on cell culture techniques, electrophoresis, microscopy, enzymatic reactions, and bioinformatics. In addition to covering measurement, preparation of reagents, blotting, chromatography, and real-time PCR, Current Protocols Essential Laboratory Techniques also:
- Teaches new investigators how to perform basic laboratory research techniques
- Explains solution chemistry and preparation
- Describes care and use of common equipment such as pH meters, spectrophotometers, centrifuges, and microscopes
- Covers basic laboratory safety
- Provides access to Current Protocols Essential Laboratory Techniques online
Written by distinguished investigators and outstanding faculty, Current Protocols Essential Laboratory Techniques, Second Edition is a great source for beginning scientists who want to develop the skills for a fruitful career in research.
List of contents
Foreword Preface
Contributors List
Conversion Factors
Flow Chart: Application of Techniques
Emily Wiley, Claremont Colleges Chapter 1 Volume/Weight Measurement Unit 1.1 Volume Measurement
Andrew Zanella and Tom Davis, Claremont Colleges Unit 1.2 Weight Measurement
Michael Guzy, Ohaus, Corp. Chapter 2 Concentration Measurement Unit 2.1 Spectrophotometry
Rob Morris, Ocean Optics, Inc. Unit 2.2 Quantitation of DNA and RNA with Absorption and Fluorescence Spectroscopy
Sean R. Gallagher, UVP, LLC Chapter 3 Reagent Preparation Unit 3.1 Theoretical Discussion
Deb Chakravarti, Keck Graduate Institute Unit 3.2 Practical Discussion
Deb Chakravarti, Keck Graduate Institute Unit 3.3 Recipes In-House Chapter 4 Cell Culture Techniques Unit 4.1 Aseptic Technique
Thomas Bykowski and Brian Stevenson, University of Kentucky College of Medicine Unit 4.2 Culture of Escherichia coli and Related Bacteria
Sean P. Riley, Michael E. Woodman, and Brian Stevenson, University of Kentucky College of Medicine Chapter 5 Sample Preparation Unit 5.1 Centrifugation and Cell Fractionation In-House Unit 5.2 Purification and Concentration of Nucleic Acids
Dennis H. Dowhan, University of Queensland Chapter 6 Chromatography Unit 6.1 Overview of Chromatography
Buddhadeb Mallik, Bulbul Chakravarti, and Deb N. Chakravarti, Keck Graduate Institute of Applied Life Sciences Unit 6.2 Column Chromatography
Deb Chakravarti, Keck Graduate Institute Chapter 7 Electrophoresis Unit 7.1 Overview of Electrophoresis
Sean R. Gallagherr, UVP, LLC Unit 7.2 Agarose Gel Electrophoresis
Jennifer A. Armstrong, The Claremont Colleges, and Joseph R. Schulz, Occidental College Unit 7.3 SDS-PAGE
Sean Gallagher, UVP, LLC Unit 7.4 Staining of Gels
Sean Gallagher, UVP, LLC Chapter 8 Blotting Unit 8.1 Overview of Blotting
Maria Cristina Negritto, Pomona College, and Glenn M. Manthey, City of Hope, Beckman Research Institute Unit 8.2 Nucleic Acid Blotting: Southern and Northern
Laura L. Mays Hoopes, Pomona College Unit 8.3 Protein Blotting: Immunoblotting
Sean Gallagher, UVP, LLC Unit 8.4 Labeling DNA and Probe Preparation
Karl A. Haushalter, Harvey Mudd College Chapter 9 Microscopy Unit 9.1 Conventional Light Microscopy
Eric S. Cole, St. Olaf College Unit 9.2 Immunofluorescence Microscopy
David J. Asai, Harvey Mudd College Chapter 10 Enzymatic Reactions Unit 10.1 Working with Enzymes
David Skrincosky and Cindy Santangelo, Worthington Biochemical Corp. Unit 10.2 Overview of PCR
Christine D. Kuslich, Molecular Profiling Institute, Buena Chui, GE Healthcare, and Carl T. Yamashiro, Molecular Profiling Institute Unit 10.3 Real-Time PCR
Dean Fraga, College of Wooster, Tea Meulia, Ohio Agricultural Research and Development Center, Steven Fenster, Ashland University Chapter 11 Bioinformatics Unit 11.1 Using NCBI Blast
Nicholas A. Stover, Bradley University, and Andre R.O. Cavalcanti, Pomona College
About the author
Sean R. Gallagher, Ph.D. is the Chief Technology Officer and Director of Product Development for UVP, Inc. (Upland, CA), where he oversees applied technology and product development for specialty light source and life science products. He is on the faculty of the Keck Graduate Institute of Applied Life Sciences and Harvey Mudd College (Claremont, CA) and holds eight patents on technology he developed. He received his doctorate in Botany from the University of California at Riverside and completed a post-doctoral position at Stanford University.
Emily A. Wiley, Ph.D. is an Associate Professor of Biology in the Joint Science Department of the Claremont-McKenna, Scripps, and Pitzer Colleges, Claremont, CA. She received her doctorate in Molecular Genetics from the University of Washington. She is actively involved in graduate and undergraduate teaching courses and is committed to providing proper training to those students. She has an active research program studying chromatin assembly in
Tetrahymena thermophila.
Summary
Provides students with the biological laboratory techniques needed to pursue any major in the life and health sciences. This title gives researchers the skills and understanding of the fundamental laboratory procedures that are needed to run experiments, solve problems, and become a productive member of the modern life science laboratory.
