Lectins - Methods and Protocols

Lectins: Methods and Protocols summarizes classic lectin technologies and advanced techniques with high throughputs and sensitivities. Chapters include methods and techniques for serial lectin-affinity chromatography procedure, lectin-probed western blot and histochemical analyses, quantitative interaction analyses based on equilibrium dialysis, isothermal calorimetry, surface plasmon resonance, techniques for elucidating functions of endogenous animal lectins, advanced methods on engineer novel lectins by evolutionary concepts, and special equipment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Lectins: Methods and Protocols seeks to aid not only lectin specialists but also non-experts including both young scientists.

List of contents

PART I: STANDARD TECHNIQUES

1. History of Plant Lectin Research
Els J.M. Van Damme

2. Fungal Lectins, A Growing Family
Yuka Kobayashi, and Hirokazu Kawagishi

3. The "White Kidney Bean Incident" in Japan
Haruko Ogawa, and Kimie Date

4. Hemagglutination (Inhibition) Assay
Kotone Sano, and Haruko Ogawa

5. Preparation of Affinity Adsorbents and Purification of Lectins from Natural Sources
Kimie Date and Haruko Ogawa

6. High-Performance Lectin Affinity Chromatography
Yuka Kobayashi

7. Determination of Glycan Motifs Using Serial Lectin Affinity Chromatography
Katsuko Yamashita, and Takashi Ohkura

8. Lectin-Probed Western Blot Analysis
Takeshi Sato

9. Solid-Phase Assay of Lectin Activity Using HRP-conjugated Glycoproteins
Kyoko Kojima-Aikawa

10. A Simple Viral Neuraminidase-based Detection for High-throughput Screening of Viral Hemagglutinin-host Receptor Specificity
Nongluk Sriwilaijaroen, and Yasuo Suzuki

11. Lectin Affinity Electrophoresis
Yuka Kobayashi

12. Capillary-Based Lectin Affinity Electrophoresis for Interaction Analysis between Lectins and Glycans
Mitsuhiro Kinoshita, and Kazuaki Kakehi

13. Basic Procedures for Lectin Flow Cytometry
Kenta Moriwaki, and Eiji Miyoshi

14. Histochemical Staining Using Lectin Probes
Yoshihiro Akimoto, and Hayato Kawakami

15. Equilibrium Dialysis using Chromophoric Sugar Derivatives
Tomomitsu Hatakeyama

16. Centrifugal Ultrafiltration-HPLC Method for Interaction Analysis between Lectins and Sugars
Kanji Hori and Makoto Hirayama

17. Surface Plasmon Resonance as a Tool to Characterize Lectin-carbohydrate Interactions
Yasuro Shinohara, and Jun-ichi Furukawa

18. Isothermal Calorimetric Analysis of Lectin-sugar Interaction
Yoichi Takeda and Ichiro Matsuo

19. Carbohydrate-Lectin Interaction Assay by Fluorescence Correlation Spectroscopy Using Fluorescence-Labeled Glycosylasparagines
Mamoru Mizuno


PART II: EMERGING TECHNIQUES FOR LECTIN-BASED GLYCOMICS

20. Lectin-Based Glycomics: How and When Was the Technology Born?
Jun Hirabayashi

21. Frontal Affinity Chromatography (FAC): Theory and Basic Aspects
Ken-ichi Kasai

22. Frontal Affinity Chromatography: Practice of Weak Interaction Analysis Between Lectins and Fluorescently Labeled Oligosaccharides
Chihiro Sato

23. Differential Glycan Analysis of an Endogenous Glycoprotein: Toward Clinical Implementation. From Sample Pretreatment to Data Standardization
Atsushi Kuno, Atsushi Matsuda, Sachiko Unno, Binbin Tan, Jun Hirabayashi, and Hisashi Narimatsu

24. Lectin-Microarray Technique for Glycomic Profiling of Fungal Cell Surfaces
Azusa Shibazaki, and Tohru Gonoi

25. Application of Lectin Microarray to Bacteria Including Lactobacillus casei /paracasei Strains
Emi Yasuda, Tomoyuki Sako, Hiroaki Tateno, and Jun Hirabayashi

26. Live Cell Imaging of Human Pluripotent Stem Cells by a Novel Lectin Probe rBC2LCN
Hiroaki Tateno, Yasuko Onuma, and Yuzuru Ito

27. Carbohydrate-binding Specificity of Lectins Using Multiplexed Glyco-bead Array
Kazuo Yamamoto

28. Supported Molecular Matrix Electrophoresis: A New Membrane Electrophoresis for Characterizing Glycoproteins
Yu-ki Matsuno, and Akihiko Kameyama


PAPRT III: TECHNIQUES FOR ELUCIDATING FUNCTIONS OF ENDOGENOUS ANIMAL LECTINS

29. Overall Strategy for Functional Analysis of Animal Lectins
Norihito Kawasaki

30. Evaluation of Glycan-Binding Specificity by Glycoconjugate Microarray with an Evanescent-Field Fluorescence Detection System
Hiroaki Tateno

31. Potential Usage for in vivo Lectin Screening in Live Animals Utilized by Cell Surface Mimetic Glyco-nanoparticles, Phosphorylcholine-Coated Quantum Dots (PC-QDs)
Maho Amano, Hiroshi Hinou, Risho Miyoshi, and Shin-Ichiro Nishimura

32. Remodeling Cell Surface Glycans using Glycosylation Inhibitors
Norihito Kawasaki

33. Remodeling of Glycans Using Glycosyltransferase Genes
Yuko Naito-Matsui, and Hiromu Takematsu

34. Functional Assay Using Lectin Gene Targeting Technologies (Over-Expression)
Motohiro Nonaka, and Toshisuke Kawasaki

35. Analysis of L-selectin-mediated Cellular Interactions under Flow Conditions
Hiroto Kawashima

36. Assessment of weak sugar-binding ability using lectin tetramer and membrane-based glycans
Kazuo Yamamoto


PART IV: STRUCTURAL BIOLOGY AND ENGINEERING OF LECTINS

37. Perspectives in Glycomics and Lectin Engineering
Jan Tkac, Tomas Bertok, Jozef Nahalka, and Peter Gemeiner

38. Molecular Basis of Pandemic of Avian-Type Influenza Virus
Nongluk Sriwilaijaroen, and Yasuo Suzuki

39. Basic Procedure of X-ray Crystallography for Analysis of Lectin-sugar Interactions
Zui Fujimoto

40. A New Structure Determination Method of Lectins Using a Selenium-containing Sugar Ligand
Hisayoshi Makyio, and Ryuichi Kato

41. NMR Analysis of Carbohydrate-Binding Interactions in Solution: An Approach Using Analysis of Saturation Transfer Difference NMR Spectroscopy
Hikaru Hemmi

42. Small Angle X-ray Scattering to Obtain Models of Multivalent Lectin-Glycan Complexes
Stephen D. Weeks, and Julie Bouckaert

43. Directed Evolution of Lectins by an Improved Error-prone PCR and Ribosome Display Method
Dan Hu, Hiroaki Tateno, and Jun Hirabayashi

44. Tracing Ancestral Specificity of Lectins: Ancestral Sequence Reconstruction Method as a New Approach in Protein Engineering
Tomohisa Ogawa, and Tsuyoshi Shirai


PART V: COMPREHENSIVE LISTS

45. Comprehensive List of Lectins: Origins, Natures, and Carbohydrate Specificities
Yuka Kobayashi, Hiroaki Tateno, Haruko Okawa, Kazuo Yamamoto, and Jun Hirabayashi

46. Lectin Structures: Classification Based on the 3-D Structures
Zui Fujimoto, Hiroaki Tateno, and Jun Hirabayashi

Summary

Lectins: Methods and Protocols summarizes classic lectin technologies and advanced techniques with high throughputs and sensitivities. Chapters include methods and techniques for serial lectin-affinity chromatography procedure, lectin-probed western blot and histochemical analyses, quantitative interaction analyses based on equilibrium dialysis, isothermal calorimetry, surface plasmon resonance, techniques for elucidating functions of endogenous animal lectins, advanced methods on “engineer” novel lectins by evolutionary concepts, and special equipment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Lectins: Methods and Protocols seeks to aid not only lectin specialists but alsonon-experts including both young scientists.

Product details

• Assisted by: Ju Hirabayashi (Editor), Jun Hirabayashi (Editor)
• Publisher: Springer, Berlin
• Languages: English
• Product format: Hardback
• Released: 14.05.2014
• EAN: 9781493912919
• ISBN: 978-1-4939-1291-9
• No. of pages: 613
• Dimensions: 183 mm x 29 mm x 260 mm
• Weight: 1439 g
• Illustrations: XIII, 613 p. 168 illus., 60 illus. in color.
• Series: Methods in Molecular Biology; Humana Press; Methods in Molecular Biology
• Subject: Natural sciences, medicine, IT, technology > Biology > General, dictionaries