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Cardiac Tissue Engineering: Methods and Protocols presents a collection of protocols on cardiac tissue engineering from pioneering and leading researchers around the globe. These include methods and protocols for cell preparation, biomaterial preparation, cell seeding, and cultivation in various systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Cardiac Tissue Engineering: Methods and Protocols highlights the major techniques, both experimental and computational, for the study of cardiovascular tissue engineering.
List of contents
Second Generation Codon Optimized Minicircle (Comic) For Non-Viral Reprogramming Of Human Adult Fibroblasts.- Scalable Cardiac Differentiation Of Human Pluripotent Stem Cells as Microwell-Generated, Size Controlled Three-Dimensional Aggregates.- Preparation And Characterization Of Circulating Angiogenic Cells For Tissue Engineering Applications.- Isolation And Expansion Of C-Kit Positive Cardiac Progenitor Cells By Magnetic Cell Sorting.- Synthesis Of Aliphatic Polyester Hydrogel For Cardiac Tissue Engineering.- Fabrication Of Pegylated Fibrinogen, A Versatile Injectable Hydrogel Biomaterial.- Native Cardiac Extracellular Matrix Hydrogels For Cultivation Of Human Stem Cell-Derived Cardiomyocytes.- Magnetically-Actuated Alginate Scaffold: A Novel Platform For Promoting Tissue Organization And Vascularisation.- Shrink-Induced Biomimetic Wrinkled Substrates For Functional Cardiac Cell Alignment and Culture.- Injectable ECM Scaffolds For Cardiac Repair.- Generation Of Strip-Format Fibrin-Based Engineered Heart Tissue (EHT).- Cell Tri-Culture For Cardiac Vascularization.- Cell Sheet Technology For Cardiac Tissue Engineering.- Design And Fabrication Of Biological Wires.- Collagen-Based Engineered Heart Muscle.- Creation Of A Bioreactor For The Application Of Variable Amplitude Mechanical Stimulation Of Fibrin-Gel Based Engineered Cardiac Tissue.- Preparation Of Acellular Myocardial Scaffolds With Well-Preserved Cardiomyocyte Lacunae, And Method For Applying Mechanical And Electrical Simulation To Tissue Construct.- Patch-Clamp Technique In ESC-Derived Cardiomyocytes.- Optogenetic Control Of Cardiomyocytes.- Methods For Assessing The Electromechanical Integration Of Human Pluripotent Stem Cell-Derived Cardiomyocyte Graft.- Quantifying Electrical Interactions Between Cardiomyocytes and Other Cells In Micropatterned Cell Pairs.
Summary
Cardiac Tissue Engineering: Methods and Protocols presents a collection of protocols on cardiac tissue engineering from pioneering and leading researchers around the globe. These include methods and protocols for cell preparation, biomaterial preparation, cell seeding, and cultivation in various systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Cardiac Tissue Engineering: Methods and Protocols highlights the major techniques, both experimental and computational, for the study of cardiovascular tissue engineering.
