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This translation from Russian into English of the monograph Hematopoi etic and Lymphoid Tissue in Culture by E. A. Luriya provides us for the first time with a comprehensive review of the massive amount of work carried out in the USSR in this important area of biological research. Historically, the pioneering tissue culture studies of Maksimov, Popov, Chasovnikov, and their colleagues working in Russia in the early part of this century provided much of the impetus for subsequent studies on lymphopoiesis and hematopoiesis in vitro and much of the basic information used subsequently to achieve immune responses in vitro and to develop the methodology for cloning hematopoietic stem cells. Particularly welcome in this translation is the addition of the Appendix, in which Drs. Luriya and Fridenshtein describe in some detail the methodology that has been developed in their laboratories for the study of a variety of cell types in cell and tissue culture. For many of us, this description provides information that had previously not been accessible to us: porosity of millipore fIlters in relation to growth properties; colony-forming cell adhesiveness; pro liferation kinetics of stromal cells; differential effectiveness of various media used for culture of liver cells; details of washing procedures for fIlter substrates used in culture.
List of contents
The Study of Hematopoietic and Lymphoid Tissues in Tissue Culture.- The Development of Tissue Culture Methods.- I. Hematopoietic Tissue In Vitro.- 1. Hematopoietic Tissue in Plasma Clot Culture.- 2. Monolayer Cultures of Hematopoietic Tissue.- 3. Suspension Cultures of Hematopoietic Tissue.- 4. Formation of Hematopoietic Foci in Agar Cultures.- 5. Organ Cultures of Hematopoietic Tissue.- 6. A Comparative Assessment of the Various Methods of Hematopoietic Tissue Culture.- 7. Factors That Influence Differentiation of Hematopoietic Tissue In Vitro.- II. Possibilities of Differentiation of Cells Grown in Hematopoietic Tissue Cultures on Retransplantation In Vivo.- III. Lymphoid Tissue In Vitro.- 1. Lymphoid Tissue Explanted in a Plasma Clot.- 2. Monolayer Cultures of Lymphoid Tissue and Peripheral Blood Cells.- 3. Suspension Cultures of Lymphoid Tissue and Peripheral Blood Cells.- 4. Organ Cultures of Lymphoid Tissue.- 5. Comparison of Various Methods of Lymphoid Tissue Culture.- IV. Immunological Functions of Lymphoid Tissue In Vitro.- 1. The Process of Antibody Formation.- 2. The Delayed Hypersensitivity Reaction In Vitro.- V. Cell Lines in Lymphoid and Hematopoietic Tissue.- Conclusion.- Appendix. Recent Experience in Monolayer and Organ Cultures of Hematopoietic Tissue - E. A. Luriya and A. Ya. Fridenshtein.- 1. The Technique of Cloning Fibroblasts in Monolayer Cultures of Hematopoietic and Lymphoid Cells and Its Use to Study Stromal Precursor Cells.- 2. The Method of Multiple Organ Cultures of Hematopoietic and Lymphoid Tissue.- References.
