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This book describes the principles and reactions for most techniques of protein extraction, purification, and characterization. The second edition covers modern techniques that are extensively used in protein identification and analyses including NMR, mass spectrometry, recombinant protein, and database search. It incorporates NMR and mass spectrometry for protein identification, use of computers and websites for protein annotation, and identification of commonly modified proteins. The text also expands coverage of the recombinant proteins. Two new chapters address the identification and annotation of the target protein and the identification of other posttranslational modifications.
List of contents
Extraction of Protein. Estimation of Protein. Electrophoretic Analyses of Protein. Purification of Protein. Antibodies: Structures, Interactions, and Production. Antibody Labeling, Antibody Detection, and Immunochemical Techniques. Identification and Annotation of the Target Protein. Identification of Most Common Post-translational Modification (Glycosylation): Purification of Glycoproteins and Analyses of Their Oligosaccharides. Identification of Other Posttranslational Modifications.
About the author
Hafiz Ahmed is an Assistant Professor at the University of Maryland Biotechnology Institute, Center of Marine Biotechnology, Baltimore.
Summary
This book describes the principles and reactions for most techniques of protein extraction, purification, and characterization. The second edition covers modern techniques that are extensively used in protein identification and analyses including NMR, mass spectrometry, recombinant protein, and database search. It incorporates NMR and mass spectrometry for protein identification, use of computers and websites for protein annotation, and identification of commonly modified proteins. The text also expands coverage of the recombinant proteins. Two new chapters address the identification and annotation of the target protein and the identification of other posttranslational modifications.
Key selling features:
- Features new chapters on the identification and annotation of the target protein and the identification of other posttranslational modifications
- Incorporates NMR and mass spectrometry for protein identification, use of computers and web sites for protein annotation, and identification of commonly modified proteins
- Expands coverage of the recombinant proteins (preparation, extraction, and purification including His-tagged columns)
- Describes the principles and reactions for most techniques of protein extraction, purification, and characterization
- Provides background knowledge on most protein techniques with advantages and disadvantages and useful advice on their applications
