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Klappentext The aim of the book is to introduce the reader to the kinetic analysis of a wide range of biological processes at the molecular level. It is intended to show that the same approach can be used to resolve the number of steps in enzyme reactions, muscle contraction, visual perception and ligand binding receptors that trigger other physiological processes. Attention is also given to methods for characterizing these steps in chemical terms. Although the treatment is mainly theoretical, a wide range of examples and experimental techniques are also introduced and an historical approach is used to demonstrate the development of the theory and experimental techniques of kinetic analysis in biology. Zusammenfassung The aim of the book is to introduce the reader to the kinetic analysis of a wide range of biological processes at the molecular level. It is intended to show that the same approach can be used to resolve the number of steps in many types of biological processes. Inhaltsverzeichnis Part I. The Time Scales of Nature: a Historical Survey: 1. The history of the application of physical principles; 2 Kinetics and biological problems; 3 Time scales of nature and of measurement; Part II. Some Mathematical Introductions: 4. The origins of exponential behaviour; 5. Modelling of kinetic systems; 6. Data collection, reduction and analysis; 7. Guidelines for the derivation of equations used in the text; Part III. Elementary Kinetic Equations: Ground Rules: 8. The law of mass action, rates and equilibria; 9. The evaluation of first and second order rate constants; 10. An introduction to practical steady state kinetics; 11. Steady state kinetics of reversible effectors and ionic equilibria; Part IV. Kinetic Analysis of Complex Reactions: 12. Introduction to the study of transients and reaction sequences; 13. Consecutive reactions and physiological responses; Part V. Kinetic Mechanisms of Enzyme Reactions: 14. Transient kinetics and enzyme reactions; 15. Sequential enzyme reactions in metabolism and control; 16. Kinetics of ligand dissociation and exchange between binding sites; Part VI. Chemical Relaxation Phenomena: 17. Applications of relaxation kinetics; 18. Basic equations for the analysis of chemical relaxations; 19. Chemical relaxation of ligand binding and recognition; 20. Relaxations of proton equilibria; PartVII. Factors Affecting the Rates of Chemical Reactions; 21. Introduction to the history and concepts of chemical dynamics; 22. Temperature dependence of reactions and the activation parameters; 23. Viscosity and molecular dynamics; 24. Diffusion control of reactions; 25. Some comments on the use of isotopes in kinetic investigations; Part VIII. The Role of Light in Kinetic Investigations; 26. Applications of photochemistry; 27. Luminescence and other excited states; 28. The scope of fluorescence life time studies; 29. Photolabile (caged) compounds for the initiation of reactions; References; Index....
