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A comprehensive collection of classic and innovative methodologies used in many laboratories for the investigation of multiple myeloma. These readily reproducible techniques range from the standard Plasma Cell Labeling Index methodology to a final chapter on making sense of microarrays, and include the full spectrum of cytogenetic and molecular diagnostic methods. The protocols follow the successful Methods in Molecular Medicine(TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. These proven techniques are ideal for studying the pathogenesis of multiple myeloma and identifying new therapeutic targets.
List of contents
Multiple Myeloma.- Immunophenotyping of Plasma Cells in Multiple Myeloma.- Plasma Cell Labeling Index.- Conventional Cytogenetics in Myeloma.- Multicolor Spectral Karyotyping in Multiple Myeloma.- Detection of Chromosome 13 Deletions by Fluorescent In Situ Hybridization.- Comparative Genomic Hybridization for Analysis of Changes in DNA Copy Number in Multiple Myeloma.- Southern Blotting of IgH Rearrangements in B-Cell Disorders.- Identification and Assembly of V Genes as Idiotype-Specific DNA Fusion Vaccines in Multiple Myeloma.- Identification of Clonotypic IgH VDJ Sequences in Multiple Myeloma.- Real-Time Polymerase Chain Reaction of Immunoglobulin Rearrangements for Quantitative Evaluation of Minimal Residual Disease in Myeloma.- Incomplete DJH Rearrangements.- Identification of Malignant Plasma Cells by mRNA In Situ Hybridization.- The SCID-hu Myeloma Model.- The 5T2MM Murine Model of Multiple Myeloma.- Determination of Telomerase Activity and Telomere Length.- Dendritic Cell/Myeloma Hybrid Vaccine.- Genetically Engineered Myeloma Cell Vaccine.- An In Vitro Osteoclast-Forming Assay to Measure Myeloma Cell-Derived Osteoclast-Activating Factors.- Clonality Detection of Expanded T-Cell Populations in Patients With Multiple Myeloma.- Detecting Mismatch Repair Defects in Myeloma.- Methylation-Specific Polymerase Chain Reaction.- Making Sense of DNA Microarray Data.
Summary
A comprehensive collection of classic and innovative methodologies used in many laboratories for the investigation of multiple myeloma. These readily reproducible techniques range from the standard Plasma Cell Labeling Index methodology to a final chapter on making sense of microarrays, and include the full spectrum of cytogenetic and molecular diagnostic methods. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. These proven techniques are ideal for studying the pathogenesis of multiple myeloma and identifying new therapeutic targets.
Additional text
From the reviews:
"...extremely informative...a great reference for researchers or students with an interest in the etiology and treatment of Multiple Myeloma." - The Journal of the Association of Genetic Technologists
"Audience: Hematologists, oncologists, and other clinicians … as well as biomedical researchers in the field of hematology and immunology. … The book is divided into 23 chapters, including schematic diagrams, figures, color plates, and tables, as well as an index of important terms at the end. … covers clinical as well as experimental aspects of multiple myeloma and provides an important set of information for clinicians and researchers, in different fields of biomedicine, including hematology, oncology, molecular diagnostic, clinical immunology, osteoimmunology, molecular-biology, genetics, and bioinformatics.” (Danka Grcevic, Croatian Medical Journal, Vol. 48, October, 2007)
Report
From the reviews:
"...extremely informative...a great reference for researchers or students with an interest in the etiology and treatment of Multiple Myeloma." - The Journal of the Association of Genetic Technologists
"Audience: Hematologists, oncologists, and other clinicians ... as well as biomedical researchers in the field of hematology and immunology. ... The book is divided into 23 chapters, including schematic diagrams, figures, color plates, and tables, as well as an index of important terms at the end. ... covers clinical as well as experimental aspects of multiple myeloma and provides an important set of information for clinicians and researchers, in different fields of biomedicine, including hematology, oncology, molecular diagnostic, clinical immunology, osteoimmunology, molecular-biology, genetics, and bioinformatics." (Danka Grcevic, Croatian Medical Journal, Vol. 48, October, 2007)
