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The first comprehensive treatise on Rapid Cycle Real-Time PCR. With amplification times of 15-30 minutes of on-line detection and analysis, nucleic acid quantification of mutation analysis finally becomes a routine, powerful and rapid method. Focusing primarily on the LightCycler, an instrument that combines Rapid Cycle PCR with fluorescent monitoring, this technology provides convenient analysis by melting temperatures. PCR products can be identified by product Tm, and single base mismatches can easily be genotyped by probe Tm. Methods chapters detail the theory behind quantification of mutation analysis; the design of synthesis of fluorescent hybridization probes of the preparation of template DNA. Application chapters apply nucleid acid quantification to infectious organisms of intracellular messengers and mutation detection to somatic of acquired mutations.
List of contents
Aus dem Inhalt:
From the contents: 1. Methods: Selection of Hybridization Probes for Real-Time Quantification and Genetic Analysis. Genotyping by Fluorescent Hybridization Probe Melting Curves. Quantification on the LightCycler
- 2. Genotyping or Human Germline Variations: High-Speed Detection of a1-Antitrypsin Deficiency Alleles Pi-S and Pi-Z on the LightCycler. High-Speed Methylenetetrahydrofolate Reductase C-T 677 Mutation Detection on the LightCycler
- 3. Acquired Genetic Alterations in Human Diseases: Quantification of Retrotransposon XIR-2.5 Copy Number in Genomes of Poeciliidae Species. Monitoring of Residual Disease in Patients with Chronic Myelogenous Leukemia Using Specific Fluorescent Hybridization Probes
- 4. Receptors and Mediators: Development of Quantitative RT-PCR Tests for the Expression of Cytokine Genes
- 5. Infectious Organisms: Quantitative Detection of Hepatitis B Virus DNA
- 6. Plant Gene Products: Quantification of Genetically Modified Soybeans in Food
