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To prepare synchronized cells representing different stages of the cell cycle has been a great challenge for researchers across the globe. In Cell Cycle Synchronization: Methods and Protocols, experts in the field supply detailed protocols providing first the theoretical background of the procedure then step-by-step instructions on how to implement synchronization as well as the latest techniques for the enhanced study of regulatory mechanisms to understand cell cycle events. Describing synchronized cells from asynchronous bacterial, plant, protozoan, yeast, fish, and mammalian cell cultures, the synchronization methods presented in the book are based principally on two major strategies: the "arrest-and-release" approach, which involves different chemical treatments to block cells at certain stages of the cell cycle, and the physical strategy, which contains physical methods to collect cells belonging to subpopulations of the cell cycle. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Detailed and easy to follow, Cell Cycle Synchronization: Methods and Protocols is an ideal guide for scientists who wish to make use of these powerful synchronization techniques but have no access to thorough, time-tested protocols designed for doing so.
List of contents
Overview of Cell Synchronization.- Synchronization of Mammalian Cells and Nuclei by Centrifugal Elutriation.- Cytofluorometric Purification of Diploid and Tetraploid Cancer Cells.- Large Scale Mitotic Cell Synchronization.- Synchronization of Mammalian Cell Cultures by Serum Deprivation.- Cell Synchronization by Inhibitors of DNA Replication Induces Replication Stress and DNA Damage Response: Analysis by Flow Cytometry.- Chromosome Formation during Fertilization in Eggs of the Teleost, Oryzias latipes.- Specific Cell Cycle Synchronization with Butyrate and Cell Cycle Analysis.- Analysis of Nuclear Uracil DNA-Glycosylase (nUDG) Turnover during the Cell Cycle.- Synchronization of HeLa Cells.- Synchronization of Bacillus subtilis Cells by Spore Germination and Outgrowth.- Synchronization of Yeast.- Synchronization of Pathogenic Protozoans.- Synchronization of In Vitro Maturation in Porcine Oocytes.- Synchronization of Medicago sativa Cell Suspension Culture.- Cell Cycle Synchronization for the Purpose of Somatic Cell Nuclear Transfer (SCNT).- Ex Vivo Expansion of Hematopoietic Stem Cells to Improve Engraftment in Stem Cell Transplantation.- Flow Cytometry Developments and Perspectives in Clinical Studies: Examples in ICU Patients.- Molecular Network Dynamics of Cell Cycle Control: Transitions to Start and Finish.
Summary
This Methods in Molecular Biology(TM) volume offers detailed protocols, with theoretical background, and step-by-step instructions on implementing synchronization, as well as current techniques for the enhanced study of regulatory mechanisms in cell cycle events.
