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The 18 chapters making up In Vitro Haploid Production in Higher Plants are divided into two sections. Section 1 (eight chapters) covers historical and fundamental aspects of haploidy in crop improvement. Section 2 deals with methods of haploid production, including anther culture, micropore culture, ovary culture, pollination with irradiated pollen, in vitro pollination, and special culture techniques, including polyhaploid production in the Triticeae by sexual hybridization, the influence of ethylene and gelling agents on anther culture, conditional lethal markers, and methods of chromosome doubling.
List of contents
I1. The discovery of anther culture technique for the production of haploids A personal reflection.- I.2. Haploids in plant breeding.- I.3. Reflections on doubled haploids in plant breeding.- I.4. Cytogenetics and potential of haploidy in forest tree genetics and improvement.- I.5. Haploidy and mutation techniques.- I. 6. Cytological and biochemical aspects of in vitro androgenesis in higher plants.- I.7. Molecular and biochemical events during the induction of microspore embryogenesis.- I.8. The utility of doubled haploid populations for studying the genetic control of traits determined by recessive alleles.- I.9. Anther culture.- I.10. Influence of ethylene in microspore embryogenesis.- I.11. Effect of gelling agents on anther cultures.- I.12. Microspore culture.- I.13. Ovary and ovule culture for haploid production.- I.14. In vitro pollination and fertilization.- I.15. Irradiated pollen for haploid production.- I.16. Polyhaploid production in the Triticeae by sexual hybridization.- I.17. Conditional lethal markers: spontaneous haploid selection in plants.- I.18. Methods to double haploid chromosome numbers.- List of Contributors.- Species and Subject index.
Report
`It should be an invaluable reference for researchers interested in theoretical and practical aspects of haploidy in higher plants. Plant cytogeneticists, geneticists, plant brreeders, and biotechnologists will find this book very useful.'
Journal of Heredity (March 1999)
