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Caspases, Paracaspases, and Metacaspacses: Methods and Protocols is a collection of laboratory protocols covering current methods that are employed to measure and detect activities of these proteases in diverse biological systems, ranging from unicellular organisms to mammals. Broken into two parts, the first part focuses on methods to measure, detect, and inhibit activation and activity of a subset of or specific caspases in vitro and in several model systems and organisms, primarily in the context of programmed cell death. The second part of the book provides experimental protocols for purification and in vitro and in vivo analysis of yeast, protozoan and plant metacaspases, as well as of a human paracaspase MALT1. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.
Authoritative and practical, Caspases, Paracaspases, and Metacaspacses: Methods and Protocols seeks to aid scientists easy-to-follow techniques.
List of contents
General in vitro Caspase Assay Procedures.- Positional Scanning Substrate Combinatorial Library (PS-SCL) Approach to Define Caspase Substrate Specificity.- Global Identification of Caspase Substrates using PROTOMAP (PROtein TOpography and Migration Analysis Platform).- Caspase-2 Protocols.- Caspase-14 Protocols.- Caspase Protocols in Caenorhabditis elegans.- Detecting Caspase Activity in Drosophila Larval Imaginal Discs.- Methods for the Study of Caspase Activation in the Xenopus laevis Oocyte and Egg Extract.- Caspase Protocols in Mice.- Measurement of Caspase Activation in Mammalian Cell Cultures.- Detection and Measurement of Paracaspase MALT1 Activity.- Leishmania Metacaspase: an Arginine-Specific Peptidase.- Purification, Characterization, and Crystallisation of Trypanosoma Metacaspases.- Monitoring the Proteostasis Function of the Saccharomyces cerevisiae Metacaspase Yca1.- Plant Metacaspase Activation and Activity.- Preparation of Arabidopsis thaliana Seedling Proteomes for Identifying Metacaspase Substrates by N-terminal COFRADIC.
Summary
Caspases, Paracaspases, and Metacaspacses: Methods and Protocols is a collection of laboratory protocols covering current methods that are employed to measure and detect activities of these proteases in diverse biological systems, ranging from unicellular organisms to mammals. Broken into two parts, the first part focuses on methods to measure, detect, and inhibit activation and activity of a subset of or specific caspases in vitro and in several model systems and organisms, primarily in the context of programmed cell death. The second part of the book provides experimental protocols for purification and in vitro and in vivo analysis of yeast, protozoan and plant metacaspases, as well as of a human paracaspase MALT1. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.
Authoritative and practical, Caspases, Paracaspases, and Metacaspacses: Methods and Protocols seeks to aid scientists easy-to-follow techniques.
