Chicken Egg Yolk Antibodies, Production and Application - IgY-Technology

Polyclonal antibodies, widely used in research and diagnostics, are conventionally isolated from the blood of immunised mammals. The fact that antibodies can also be detected in the yolk of eggs laid by immunised hens, led to the development of the yolk antibody technology as an alternative method less stressful to animals. Hens can be kept under nearly natural conditions and antibodies be isolated from the eggs.
Further, the IgY-technology has the advantage that avian antibodies can be produced against several antigens which do not induce a immune response in mammals. Protocols on how to keep and immunise hens and on the extraction, isolation and use of antibodies from yolk are described in detail in this manual.
For their work on yolk antibodies the authors received the FISEA (International Foundation for the Substitution of Animal Experimentation, Luxembourg) prize in 1997.

About the author

Michael Erhard hat an der Universität Augsburg und der Westfälischen Wilhelms-Universität Münster Politikwissenschaft, Kommunikationswissenschaft sowie Neuere und Neueste Geschichte studiert. Er hat 2003 sein Studium als Magister Artium abgeschlossen und arbeitet derzeit als PR-Berater in München.

Summary

Polyclonal antibodies, widely used in research and diagnostics, are conventionally isolated from the blood of immunised mammals. The fact that antibodies can also be detected in the yolk of eggs laid by immunised hens, led to the development of the yolk antibody technology as an alternative method less stressful to animals. Hens can be kept under nearly natural conditions and antibodies be isolated from the eggs.
Further, the IgY-technology has the advantage that avian antibodies can be produced against several antigens which do not induce a immune response in mammals. Protocols on how to keep and immunise hens and on the extraction, isolation and use of antibodies from yolk are described in detail in this manual.
For their work on yolk antibodies the authors received the FISEA (International Foundation for the Substitution of Animal Experimentation, Luxembourg) prize in 1997.

Product details

• Assisted by: Ingri Behn (Editor), Ingrid Behn (Editor), Michael Erhard (Editor), Michael Erhard et al (Editor), Andreas Hlinak (Editor), Rüdiger Schade (Editor), Christian Staak (Editor)
• Publisher: Springer, Berlin
• Languages: English
• Product format: Paperback / Softback
• Released: 14.11.2013
• EAN: 9783662044902
• ISBN: 978-3-662-04490-2
• No. of pages: 255
• Dimensions: 156 mm x 15 mm x 236 mm
• Weight: 412 g
• Illustrations: XII, 255 p. 36 illus., 3 illus. in color.
• Series: Springer Lab Manuals; Springer Lab Manual; Springer Lab Manuals
• Subjects: Natural sciences, medicine, IT, technology > Biology > Genetics, genetic engineering; B, Zellbiologie (Zytologie), Immunologie, Medical research, Immunology, Medizinische Forschung, molecular biology, biotechnology, Biomedical and Life Sciences, Cellular biology (cytology), Biomedical Research, Cell Biology, Molecular Medicine