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This publication contains proceedings of a Seminar on GLUCOSINOLATES IN RAPESEEDS - Analytical Aspects, held in Gembloux (Belgium) from 1 to 3 October 1986. The meeting was organized by request of the Commission of the European Communities in the context of the CEC Programme of Research on Plant Productivity. The main aim of the Seminar was to contribute to the elaboration of reliable quantitative methods for glucosinolate determination in rapeseeds. Fourty Experts from thirteen countries participated in this Seminar. Original contributions which were considered of special importance for the subject covered by the Seminar were presented and discussed. Thanks are due to the Chairmen, Dr. Heaney R., Dr. Biston R., Dr. Ri baillier D., Prof. Dr. Robbelen G., authors and participants in the meeting for their contributions, friendliness and cooperation. Special thanks go to Dr. Mc Gregor (Canada) and Dr. Uppstrom B. (Swe den) who gave Members the benefit of their knowledge. Finally, I would also like to thank those who have helped in organizing this Seminar Director Lecomte R. (Centre de Recherches Agronomiques de l'Etat, Gembloux), Rector Ledent A. (Faculte des Sciences Agronomiques de l'Etat, Gembloux), Prof. Severin M., Dr. Biston R., Mrs Bock and all my colleagues.
List of contents
The BCR Programme - Food Analysis.- Comparison of Myrosinase Activity Assays.- Optimization of Silylation Reactions of Desulphoglucosinolates Before Gas Chromatography.- Determination of Glucosinolates in Crucifer Vegetables for Human Consumption and Rapeseed.- Approach to Determination of HPLC Response Factors for Glucosinolates.- Isolation of Intact Glucosinolates by Column Chromatography and Determination of Their Purity.- Comparison of Procedures for Optimum Extraction. Purification and Analysis of Desulfo Indolyl Glucosinolates.- Optimization of Glucosinolates Desulphation before HPL - Chromatography.- Analysis of Individual Glucosinolates in Rapeseeds. Comparison Between Different Methods.- Quantitative Analysis of Glucosinolates in Oilseed Rape Based on HPLC of Desulphoglucosinolates and HPLC of Intact Glucosinolates.- Quick Reflectometric Determination of Total Glucosinolate Contents in Trade Samples.- Analysis of Quality Parameters of Whole Rapeseed by N.I.R.S..- Glucosinolate Analysis in Whole Rapeseed by Near Infrared Reflectance Spectroscopy.- Future Requirements for Glucosinolate Analysis - a U. K. View.- Conclusions and Recommendations.- List of Participants.
