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A wealth of information has accumulated over the last few years on the human genome. The new insights have completely changed the focus of protein analysis. It is no longer time-consuming analysis of unknown products, but rather selective identifications of individual forms, modifications and processings, and overall analysis of global protein outputs from cells and tissues in health and disease. This book gears to the rising need of sensitive, accurate, and fast separation and identification techniques in proteomics. It discusses current methodologies of modern protein analysis, from isolation and sample preparation, over analysis and identification, to final characterization. Several evaluations concentrate on the now productive approaches of two-dimensional gel electrophoresis and mass spectrometry, but alternative methods and further perspectives are also outlined. The book includes an overlook over current databases to connect protein analysis data with all available information,...
List of contents
Proteome mapping by two-dimensional polyacrylamide gel electrophoresis in combination with mass spectrometric protein sequence analysis.- Techniques for sample preparation including methods for concentrating peptide samples.- Sample preparation by SDS/PAGE and in-gel digestion.- High-resolution two-dimensional gel electrophoresis and protein identification using western blotting and ECL detection.- Nanospray mass spectrometry in protein and peptide chemistry.- MALDI-TOF mass spectrometry in protein chemistry.- The chemistry of protein sequence analysis.- The alkylated thiohydantoin method for C-terminal sequence analysis.- Ladder Sequencing.- New approaches for innovations in sensitive Edman sequence analysis by design of a wafer-based chip sequencer.- Analysis of glycoprotein-derived glycopeptides.- Lipopeptide preparation and analysis.- Phosphopeptide analysis.- Bioinformatics in protein analysis.
