Basic Cloning Procedures

This manual introduces the reader to bacic methods used in the isolation, cloning and analysis of genetic material. The protocols include RT-PCR amplification, gene cloning, hybridization analysis and sequencing of nucleic acids, PCR-based site-specific mutagenesis, analysis of protein DNA-specific interaction, cell-free protein synthesis and product electrophoretic and immunological analysis. Each protocol includes short background information, a detailed description of the necessary materials, step-by-step procedures, a troubleshooting guide and useful practical hints.

List of contents

1 cDNA Synthesis and Cloning.- 2 DNA Sequencing.- 3 RNA In Vitro Synthesis by Phage T7 DNA-Dependent RNA Polymerase.- 4 PCR-Based Site-Specific Mutagenesis.- 5 Analysis of Specific Protein-DNA Interactions.- 6 Gel Electrophoresis, Transfer and Hybridization of Nucleic Acids.- 7 Immunological Methods for Analysis of Recombinant Proteins.- 8 Protein Synthesis in Cell-Free Systems.

Product details

• Assisted by: Valdi Berzins (Editor), Valdis Berzins (Editor)
• Publisher: Springer, Berlin
• Languages: English
• Product format: Paperback / Softback
• Released: 25.07.2013
• EAN: 9783642489778
• ISBN: 978-3-642-48977-8
• No. of pages: 169
• Dimensions: 157 mm x 11 mm x 236 mm
• Weight: 284 g
• Illustrations: VII, 169 p.
• Series: Springer Lab Manuals; Springer Lab Manual; Springer Lab Manuals
• Subject: Natural sciences, medicine, IT, technology > Biology > Biochemistry, biophysics