Cytoskeleton Proteins - A Purification Manual

The standard protocols for the purification of all known cytoskeleton proteins are presented in this manual. Proteins are listed alphabetically and each protocol follows a common format. Thus, the manual provides a quick and easy reference to all relevant procedures for cytoskeleton protein purification.
The isolation procedure for each protein is shown in a clear flowchart, while the source of the protein, equipment and material needed, a list of suppliers, standard references, accession No. of sequences as well as further relevant facts and practical tips are given on a separate page.

List of contents

A General.- I User's Instructions.- II General Lab Equipment.- III Preparation of Buffers.- IV Ammonium Sulfate Precipitation.- V Chromatographic Fractionation.- VI Centrifugation.- VII Abbreviations.- B Purification Protocols.- I Actin and Actin-Associated Proteins.- II Tubulin and Microtubule-Associated Proteins.- III Motor Proteins.- IV Intermediate Filament Proteins.- V Plasmamembrane and Organelle-Associated Proteins.- C Suppliers List.

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"In one word, a perfect book, which we recommend highly, since necessary, without any exception, to all cellular and molecular laboratories, and also to the great majority of all other laboratories." Cellular and Molecular Biology

Product details

• Authors: Gerhard Isenberg
• Publisher: Springer, Berlin
• Languages: English
• Product format: Paperback / Softback
• Released: 24.07.2013
• EAN: 9783642489617
• ISBN: 978-3-642-48961-7
• No. of pages: 269
• Dimensions: 157 mm x 19 mm x 235 mm
• Weight: 427 g
• Illustrations: IV, 269 p.
• Series: Springer Lab Manuals; Springer Lab Manual; Springer Lab Manuals
• Subject: Natural sciences, medicine, IT, technology > Biology > General, dictionaries