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Including an overview of progress made in the field over the past decade, Neural Stem Cell Assays provides a detailed and comprehensive review of the basic methods for neural stem cell cultures.
List of contents
Contributors xv
Preface xix
1 Neural Differentiation of Pluripotent Stem Cells 1
Mohan C. Vemuri
1.1 Introduction 1
1.2 NSC Derivation from Rosette Formation of Embryoid Bodies 1
1.3 Rosette Free NSC in a Monolayer Culture 2
1.4 Pathways Involved in Neural Tube and Neural Crest Lineages 2
1.5 Differentiation and Gene Expression in Human Brain Development 3
1.6 Conclusions 6
References 6
2 The History and Design of Assays for the Identification and Characterization of Neural Stem Cells 9
Sharon A. Louis and Brent A. Reynolds
2.1 Introduction 9
2.2 Materials 12
2.3 Methods 14
2.4 Notes 21
Acknowledgments 23
References 23
3 Culture of Human Neural Stem Cells 25
Soojung Shin, Yiping Yan and Mohan C Vemuri
3.1 Introduction 25
3.2 Materials 25
3.3 Methods 30
3.4 Notes 33
3.5 Expected Results 33
References 34
4 GMP4-Compatible Production and Expansion of Human Neural Stem Cells 35
David J. Brick, Alexander E. Stover, Hubert E. Nethercott, Maria G. Banuelos and Philip H. Schwartz
4.1 Introduction 35
4.2 Materials 36
4.3 Methods 40
4.4 Notes 50
Acknowledgments 52
References 52
5 Primary Rat Neural Cultures 55
Navjot Kaur
5.1 Introduction 55
5.2 Materials 55
5.3 Methods 57
5.4 Notes 59
References 59
6 Cryopreservation of Human Neural Stem and Progenitor Cells 61
Profico Daniela Celeste, Sgaravizzi Giada, Projetti Pensi Massimo, Muzi Gianmarco, Ricciolini Claudia, Gelati Maurizio and Vescovi Angelo Luigi
6.1 Introduction 61
6.2 Materials 62
6.3 Methods 62
6.4 Notes 65
References 65
7 Cryopreservation and Recovery of Primary Rat Neural Cells 67
Rhonda Newman and Navjot Kaur
7.1 Introduction 67
7.2 Materials 67
7.3 Methods 68
7.4 Notes 70
References 70
8 Efficient Induction and Scale up Generation of Neural Stem Cells from Human Pluripotent Stem Cells 71
Yiping Yan, Soojung Shin and Mohan C. Vemuri
8.1 Introduction 71
8.2 Materials 71
8.3 Methods 73
8.4 Notes 78
References 79
9 Directed Differentiation of Human NSC/NPC into Dopaminergic Neurons 81
Andrzej Swistowski and Xianmin Zeng
9.1 Introduction 81
9.2 Materials 82
9.3 Methods 83
9.4 Notes 88
References 88
10 In vitro Differentiation of Pluripotent Stem Cells towards either Forebrain GABAergic or Midbrain Dopaminergic Neurons 91
Brigham J. Hartley, Bradley Watmuff, Cameron P.J. Hunt, John M. Haynes and Colin W. Pouton
10.1 Introduction 91
10.2 Materials 91
10.3 Methods 94
10.4 Notes 97
10.5 Typical Results 97
References 98
11 Generation of Astrocytes from Human Pluripotent Stem Cells using a Defined System 101
Atossa Shaltouki and Xianmin Zeng
11.1 Introduction 101
11.2 Materials and Methods 101
11.3 Notes 108
References 108
12 Directed Differentiation of Human PSC into Oligodendrocytes 111
Anu Hyysalo and Susanna Narkilahti
12.1 Introduction 111
12.2 Materials 111
12.3 Methods 113
12.4 Notes 116
Acknowledgments 117
References 117
13 Directed Differentiation towards Human Neural Retinal Cells 119
Deepak A. Lamba
13
About the author
Navjot Kaur, Ph.D, is Staff Scientist in the Life Technologies division of the Invitrogen Corporation. His research experience covers a wide range of cell biology, protein biochemistry and molecular biology concepts, with special expertise in cell culture and cryopreservation media development, cell-based assays, primary cells, and stable cell lines.
Mohan C. Vemuri, Ph.D.,MBA is director of stem cell research and development in the Life Technologies division of Invitrogen & Applied Biosystems. The author of designs of peer-reviewed articles in the field, Dr. Vemuri is also a co-editor of three books on stem cells and stem cell cultures.
Summary
Including an overview of progress made in the field over the past decade, Neural Stem Cell Assays provides a detailed and comprehensive review of the basic methods for neural stem cell cultures.
