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At the time of the lightbulb's invention, not even the most imaginative thinkers could have predicted the prevention and treatment of disease through genes delivered by electric fields. In Electroporation Protocols: Preclinicaland Clinical Gene Medicine, top researchers provide hands-on protocols describing the process of non-viral delivery of genetic materials such as DNA, oligo-DNA, and RNA into targeted immune cells, tissues, and model animals in order to better treat disease. Following the successful Methods in Molecular Biology(TM) series format, each method and protocol offers step-by-step, readily reproducible laboratory instructions, with the benefit of several introductory reviews to aid investigators new to this exciting technology.
List of contents
Basic Concepts of DNA Transfer via Electroporation.- Application of Electroporation Gene Therapy: Past, Current, and Future.- Mechanism by Which Electroporation Mediates DNA Migration and Entry into Cells and Targeted Tissues.- Applicator and Electrode Design for In Vivo DNA Delivery by Electroporation.- Electrode Assemblies Used for Electroporation of Cultured Cells.- Formulations for DNA Delivery via Electroporation In Vivo.- Overview of Drug Delivery and Alternative Methods to Electroporation.- Nanoelectroporation: A First Look.- In Vitro Targeted Gene Delivery via Electroporation.- Nucleofection of Human Embryonic Stem Cells.- Delivery of Whole Tumor Lysate into Dendritic Cells for Cancer Vaccination.- Delivery of Tumor-Antigen-Encoding mRNA into Dendritic Cells for Vaccination.- Delivery of DNA into Natural Killer Cells for Immunotherapy.- Electroporation of Adherent Cells In Situ for the Study of Signal Transduction and Gap Junctional Communication.- Delivery of DNA into Adipocytes within Adipose Tissue.- In Vivo Targeted Gene Delivery via Electroporation.- Delivery of DNA into Muscle for Treating Systemic Diseases: Advantages and Challenges.- Delivery of DNA into Skeletal Muscle in Large Animals.- Delivery of DNA into Skin via Electroporation.- Electroporation-Mediated Gene Delivery to the Lungs.- Delivery of DNA into Bladder via Electroporation.- Analysis of Gene Function in the Retina.- Optical In Vivo Imaging of Electrically Mediated Delivery of siRNA into Muscle for Gene Function Analysis.- Electroporation of Adult Zebrafish.- Treatment of Cancer via Electroporation Gene Therapy.- Flow Electroporation with Pulsed Electric Fields for Purging Tumor Cells.- Delivery of DNA into Tumors.- Intratumoral Bleomycin and IL-12 Electrochemogenetherapy for Treating Head and Neck Tumors in Dogs.- Systemic IL-12 Gene Therapy for Treating Malignancy via Intramuscular Electroporation.- Treatment of SCCVII Tumors with Systemic Chemotherapy and Interleukin-12 Gene Therapy Combination.- Electroporation for Drug and Gene Delivery in the Clinic: Doctors Go Electric.- IL-2 Plasmid Electroporation: From Preclinical Studies to Phase I Clinical Trial.- Treatment of Other Diseases via Electroporation Gene Therapy.- Factor IX Gene Therapy for Hemophilia.- KGF-1 for Wound Healing in Animal Models.- Hepatocyte Growth Factor Gene Therapy for Hypertension.- Electroporation of Corrective Nucleic Acids (CNA) In Vivo to Promote Gene Correction in Dystrophic Muscle.- Gene Delivery to Dystrophic Muscle.- Effect of Electroporation on Cardiac Electrophysiology.- Muscle and Fat Mass Modulation in Different Clinical Models.- Applications of Electroporation for DNA Vaccination.- DNA Vaccination for Prostate Cancer.- HER2/neu DNA Vaccination for Breast Tumors.- Electroporation-Mediated HBV DNA Vaccination in Primate Models.- Taking Electroporation-Based Delivery of DNA Vaccination into Humans: A Generic Clinical Protocol.- Production of Monoclonal Antibody by DNA Immunization with Electroporation.
Summary
At the time of the lightbulb's invention, not even the most imaginative thinkers could have predicted the prevention and treatment of disease through genes delivered by electric fields. In Electroporation Protocols: Preclinicaland Clinical Gene Medicine, top researchers provide hands-on protocols describing the process of non-viral delivery of genetic materials such as DNA, oligo-DNA, and RNA into targeted immune cells, tissues, and model animals in order to better treat disease. Following the successful Methods in Molecular Biology™ series format, each method and protocol offers step-by-step, readily reproducible laboratory instructions, with the benefit of several introductory reviews to aid investigators new to this exciting technology.
