Salmonella - Methods and Protocols

Salmonella: Methods and Protocols presents detailed methods on a variety of aspects of Salmonella research, focusing on those which provide landmarks for future discovery. Highlighted topics include molecular genotyping, rapid and accurate new methods for sensitive determination of antibiotic resistance profiles, the characterization of Salmonella-specific cell antigens, and Salmonella-host cell interactions. Detailed methods for studying Salmonella motility are also presented. Each chapter provides a short overview of the topic, which is followed by detailed explanations of techniques such as genetic manipulation and molecular imaging that offer the possibilities of using Salmonella as a therapeutic vector to interfere with disease. This is the first comprehensive volume of methods and protocols for studying Salmonella and will be indispensable to researchers engaged in the study of Salmonella, and enterobacteria in general. Because no recent comprehensive literature of this format is available on Salmonella, Salmonella: Methods and Protocols will be of value to a wide variety of researchers engaged in the study of Salmonella, and enterobacteria in general.

List of contents

Quantitative, Multiplexed Detection of Salmonella and Other Pathogens by Luminex® xMAP(TM) Suspension Array.- Detection of Salmonella by Bacteriophage Felix 01.- Physical Mapping of Salmonella Genomes.- Cloth-Based Hybridization Array System for the Identification of Antibiotic Resistance Genes in Salmonella.- Genome-Based Identification and Molecular Analyses of Pathogenicity Islands and Genomic Islands in Salmonella enterica.- Determination of the Gene Content of Salmonella Genomes by Microarray Analysis.- In Vivo Excision, Cloning, and Broad-Host-Range Transfer of Large Bacterial DNA Segments Using VEX-Capture.- Amplified Fragment Length Polymorphism Analysis of Salmonella enterica.- Salmonella Phages and Prophages-Genomics and Practical Aspects.- Salmonella Typhimurium Phage Typing for Pathogens.- Salmonella Phages Examined in the Electron Microscope.- Applications of Cell Imaging in Salmonella Research.- Analysis of Kinesin Accumulation on Salmonella-Containing Vacuoles.- Magnesium, Manganese, and Divalent Cation Transport Assays in Intact Cells.- Methods in Cell-to-Cell Signaling in Salmonella.- Development of Salmonella Strains as Cancer Therapy Agents and Testing in Tumor Cell Lines.- Further Resources for Molecular Protocols in Salmonella Research.

Summary

Salmonella: Methods and Protocols presents detailed methods on a variety of aspects of Salmonella research, focusing on those which provide landmarks for future discovery. Highlighted topics include molecular genotyping, rapid and accurate new methods for sensitive determination of antibiotic resistance profiles, the characterization of Salmonella-specific cell antigens, and Salmonella-host cell interactions. Detailed methods for studying Salmonella motility are also presented. Each chapter provides a short overview of the topic, which is followed by detailed explanations of techniques such as genetic manipulation and molecular imaging that offer the possibilities of using Salmonella as a therapeutic vector to interfere with disease. This is the first comprehensive volume of methods and protocols for studying Salmonella and will be indispensable to researchers engaged in the study of Salmonella, and enterobacteria in general. Because no recent comprehensive literature of this format is available on Salmonella, Salmonella: Methods and Protocols will be of value to a wide variety of researchers engaged in the study of Salmonella, and enterobacteria in general.

Additional text

From the reviews:

"This book presents detailed methods and protocols for the application of various analytical techniques to Salmonella research. It is aimed at geneticists, microbiologists, public health scientists, researchers and students. The book contains 17 chapters … ." (International Food Information Service, December, 2007)

"This is an interesting and very informative book that covers a broad range of methods in Salmonella research. Each chapter contains a useful introduction outlining in which field of research the methods are most likely to be useful. … Clear diagrams and photographic illustrations enrich the written text material. This book is not simply a list of laboratory methods, but it is also a valuable guide that will allow bench scientists to choose the most appropriate methodological approach to solve specific research questions." (Pietro Mastroeni, Microbiology Today, May, 2008)

"The book provides quite a wide range of protocols from different fields of Salmonella research which in most cases can also be generalized for other members of the family Enterobacteriaceae. Each chapter provides a short overview of the topic which is followed by detailed protocol description. … for those interested in entering an interesting field of Salmonella research I can recommend this book as a valuable source of a wide range of protocols enabling anybody to start from the very beginning." (I. Rychlík, Folia Microbiologia, Vol. 53 (3), 2008)

Report

From the reviews:

"This book presents detailed methods and protocols for the application of various analytical techniques to Salmonella research. It is aimed at geneticists, microbiologists, public health scientists, researchers and students. The book contains 17 chapters ... ." (International Food Information Service, December, 2007)
"This is an interesting and very informative book that covers a broad range of methods in Salmonella research. Each chapter contains a useful introduction outlining in which field of research the methods are most likely to be useful. ... Clear diagrams and photographic illustrations enrich the written text material. This book is not simply a list of laboratory methods, but it is also a valuable guide that will allow bench scientists to choose the most appropriate methodological approach to solve specific research questions." (Pietro Mastroeni, Microbiology Today, May, 2008)
"The book provides quite a wide range of protocols from different fields of Salmonella research which in most cases can also be generalized for other members of the family Enterobacteriaceae. Each chapter provides a short overview of the topic which is followed by detailed protocol description. ... for those interested in entering an interesting field of Salmonella research I can recommend this book as a valuable source of a wide range of protocols enabling anybody to start from the very beginning." (I. Rychlík, Folia Microbiologia, Vol. 53 (3), 2008)

Product details

• Authors: Heide Schatten
• Assisted by: Eisenstark (Editor), Eisenstark (Editor), Abe Eisenstark (Editor), Heid Schatten (Editor), Heide Schatten (Editor)
• Publisher: Springer, Berlin
• Languages: English
• Product format: Paperback / Softback
• Released: 26.10.2010
• EAN: 9781617376740
• ISBN: 978-1-61737-674-0
• No. of pages: 364
• Dimensions: 155 mm x 20 mm x 235 mm
• Weight: 569 g
• Illustrations: XII, 364 p. 129 illus.
• Series: Methods in Molecular Biology; Methods in Molecular Biology
• Subjects: Natural sciences, medicine, IT, technology > Biology > General, dictionaries; C, Life Sciences, INTERNAL MEDICINE, biochemistry, Gastroenterology, Infectious & contagious diseases, Pharmacology, infectious diseases, Human Genetics, Biomedical and Life Sciences, Medical Genetics, Biochemistry, general, Cell Biology, Pharmacology/Toxicology