Read more
Major advances in molecular biology, alternative RNA splicing, protein processing, identification of gene control elements, transgenic animals and bioinformatics open up multiple research avenues and allow for better understanding of neuropeptide production and function. With a selected description of techniques in neuropeptide research, Neuropeptide Methods covers aspects of design, synthesis and biological evaluation of peptides and peptide analogues. Written by leaders in the field, this book explains primary cell cultures and cell lines, as well as techniques in neuropeptide processing, trafficking and secretion. In vivo techniques quantifying blood-brain barrier permeability to small proteins in mice and developmental milestones of behavior in newborn mice are described. Neuropeptide Methods provides the reader with broad perspectives and breadth of knowledge on current topics related to neuropeptide research.
List of contents
A Short Introduction.- Preface.- Contributors.- The Design, Synthesis, and Biological Evaluation of VIP and VIP Analogs David Dangoor, Sara Rubinraut, Mati Fridkin, and Illana Gozes.- Antibody Production: Activity-Dependent Neuroprotective Protein (ADNP) as an Example Illana Gozes, Miri Holtser-Cochav, and Karin Vered.- Primary Cell Cultures and Cell Lines Inna Divinski, Inbar Pilzer, and Illana Gozes.- Transfection of DNA into Cells Inbar Pilzer, Inna Divinski, and Illana Gozes.- Transgenic Delivery and Detection of GFP in Neuropeptide Neurons J.L. Holter, J.S. Davies, P.-S. Man, T. Wells, and D.A. Carter.- Integrative Technologies for Neuropeptide Characterization Sergey E. Ilyin and Carlos R. Plata-Salamán.- Techniques in Neuropeptide Processing, Trafficking, and Secretion Niamh X. Cawley, Tulin Yanik, Irina Arnaoutova,Hong Lou, Nimesh Patel, and Y. Peng Loh.- In vivo Techniques Quantifying Blood-Brain Barrier Permeability to Small Proteins in Mice Weihong Pan, and Abba J. Kastin.- Cancer Cell Receptor Internalization and Proliferation: Effects of Neuropeptide Analogs Terry W. Moody, Michael Schumann, and Robert T. Jensen.- Developmental Milestones in the Newborn Mouse Joanna M. Hill, Maria A. Lim, and Madeleine M. Stone.
About the author
Illana Gozes is a Professor of Clinical Biochemistry, the Lily and Avraham Gildor Chair for the Investigation of Growth Factors, Head of the Dr. Diana and Zelman Elton Elbaum Laboratory for Molecular Neuroendocrinology, and a member of the Sackler Faculty of Medicince at Tel Aviv University in Israel. In addition, she is the Editor in Chief of Humana Press's groundbreaking Journal of Molecular Neuroscience.
Summary
Major advances in molecular biology, alternative RNA splicing, protein processing, identification of gene control elements, transgenic animals and bioinformatics open up multiple research avenues and allow for better understanding of neuropeptide production and function.
Additional text
From the reviews:
"Novel molecular techniques have been developed to better understand peptide function in health and disease. This book deals with the most recent protocols for investigation of peptide function. … Neuroscientists are the main audience. … With well written text and excellent diagrams, the book delves into the intricacies of peptide research, including tissue preparation, methods of DNA inoculation, and experimental mouse models of peptide research. … is a good book for neuroscience libraries." (Celso Agner, Doody’s Review Service, June, 2008)
Report
From the reviews:
"Novel molecular techniques have been developed to better understand peptide function in health and disease. This book deals with the most recent protocols for investigation of peptide function. ... Neuroscientists are the main audience. ... With well written text and excellent diagrams, the book delves into the intricacies of peptide research, including tissue preparation, methods of DNA inoculation, and experimental mouse models of peptide research. ... is a good book for neuroscience libraries." (Celso Agner, Doody's Review Service, June, 2008)
