Renal Cancer - Methods and Protocols

The purpose of Renal Cancer: Methods and Protocols is to introduce the surgeon, clinician, investigator, and research scientist to the basic methods employed in the diagnosis and treatment of renal cancer. Treatment of localized renal cancer is surgical. Treatment of metastasis with resection, radiotherapy, chemotherapy, and immunotherapy has had limited results. Therefore, new avenues of treatment are necessary. In planning this work, I have attempted to incorporate coverage by specialists from a variety of disciplines, each applying their individual expertise in renal cancer therapy. Though many of the participating authors are urologists, there are also valuable contributions from medical oncologists, laboratory inv- tigators, and pathologists. One of the inherent problems in publishing a book that describes molecular techniques, especially those techniques that are rapidly evolving, is that some of these may become obsolete after a few years. However, many of the procedures detailed here are standard approaches that have already wi- stood the test of time. Reverse transcriptase PCR, or RT-PCR, a technique that produces m- tiple copies of selected sequences of DNA, has a sensitivity of a millionfold amplication of a single cell, and thus constitutes a very precise technique for measuring the presence of tumor cells. By combining this technique with genomic hybridization, cadherin,ß and metalloproteinase expression we may then reveal other important factors in the detection, staging, aggressiveness, and treatment of this disease.

List of contents

Renal Cell Carcinoma.- Pathology of Kidney Tumors.- In Vitro Experimental Techniques.- Telomerase Assay in Renal Cancer.- Comparative Genomic Hybridization.- Analyzing the FHIT Gene by RT-PCR, Western Blotting, and Immunohistochemistry.- Detection of Apoptosis in Renal Cell Carcinoma.- Fluorescence In Situ Hybridization (FISH) to Metaphase and Interphase Chromosomes.- Polymerase Chain Reaction Detection of DNA Sequence Deletions.- Matrix Metalloproteinases and Their Inhibitors.- Purification of Growth Factor mRNA in Renal Tissues:bFGF-2, FGF-2, TGF?, and EGFR.- Laser-Capture Microdissection.- Expression of c-met and WT-1.- Molecular Analysis of the von Hippel-Lindau Disease Gene.- Experimental Models and Methods in Antibody Targeting of Renal Cell Carcinoma.- In Vivo Experimental Techniques.- Orthotopic Model of Renal Cell Carcinoma.- Murine Animal Model.- Angiogenesis Assays.- Antiangiogenic Approaches to Renal Cell Carcinoma.- Clinical Trials and Experiments.- Interferon Alpha, GM-CSF-Activated T Cells, and IL-6 in Renal Cell Carcinoma.- Immunotherapeutic Approach to Cancer with Cutaneous DNA Vaccination.- Monoclonal Antibodies.- Treatment Strategies in Metastatic Renal Cell Carcinoma.- 23 Assessment of T-Cell Immune Dysfunction in Patients with Renal Cell Carcinoma.- Transforming Growth Factor-?1 as a Novel Marker of Response to Therapy for Renal Cell Carcinoma.- Static and Flow Cytometry.

Summary

The purpose of Renal Cancer: Methods and Protocols is to introduce the surgeon, clinician, investigator, and research scientist to the basic methods employed in the diagnosis and treatment of renal cancer.

Additional text

"Virtually all potentially relevant in vitro and in vivo methodologies are addressed in this book. The sections describing the respective techniques are well written and stylistically equivalent...This book will be a useful addition to most institutional libraries. It will also be of some value to experienced investigators who already possess hands-on familiarity with a particular technique, but merely wish to adapt such methodology to renal cell cancer systems." -Doody's Health Sciences Book Review Journal

"...the intros are great! The writing style is surprisingly uniform and very readable. There is plentiful reference to clinical reality and a strong emphasis on novel treatments." -ACP News

"...the book is well written and the experimental protocols that were included are described in great detail." - Clinical Immunology

Report

"Virtually all potentially relevant in vitro and in vivo methodologies are addressed in this book. The sections describing the respective techniques are well written and stylistically equivalent...This book will be a useful addition to most institutional libraries. It will also be of some value to experienced investigators who already possess hands-on familiarity with a particular technique, but merely wish to adapt such methodology to renal cell cancer systems." -Doody's Health Sciences Book Review Journal

"...the intros are great! The writing style is surprisingly uniform and very readable. There is plentiful reference to clinical reality and a strong emphasis on novel treatments." -ACP News

"...the book is well written and the experimental protocols that were included are described in great detail." - Clinical Immunology

Product details

• Assisted by: Jac H Mydlo (Editor), Jack H Mydlo (Editor), Jack H. Mydlo (Editor)
• Publisher: Springer, Berlin
• Languages: English
• Product format: Paperback / Softback
• Released: 25.10.2010
• EAN: 9781617372087
• ISBN: 978-1-61737-208-7
• No. of pages: 403
• Weight: 606 g
• Illustrations: XII, 403 p. 55 illus.
• Series: Methods in Molecular Medicine; Methods in Molecular Medicine
• Subjects: Natural sciences, medicine, IT, technology > Medicine > Clinical medicine; C, Medicine, Oncology