Mycobacterium Tuberculosis Protocols

The aim of this book is to provide detailed protocols for studying the molecular biology of the pathogen Mycobacterium tuberculosis, and its int- actions with host cells. As established mycobacterial laboratories move - wards exploiting the genome, and laboratories with expertise in other fields apply them to mycobacteria, both traditional and novel methodologies need to be reviewed. Thus the chapters in Mycobacterium tuberculosis Protocols range from perspectives on storage of strains and safety issues to the application of the latest functional genomics technologies. The last few years have been remarkable ones for research into M. tuber- losis. The most important landmark by far has been the completion of the genome sequence of the widely studied H37Rv strain (1). We can now predict every protein and RNA molecule made by the pathogen. This information is or will soon be enriched by the addition of genome sequences of other strains from the M. tuberculosis complex: a second strain of M. tuberculosis, My- bacterium bovis, and the vaccine strain, M. bovis BCG. Valuable comparative data will also be provided by the genome sequences of Mycobacterium leprae, Mycobacterium avium, and Streptomyces coelicolor. Another recent milestone for M. tuberculosis has been the development of efficient mutagenesis me- odologies, the lack of which has been a major handicap in functional studies.

List of contents

Plasmid Vectors.- Isolation of DNA from Mycobacterium tubercolosis.- Extraction of RNA from Intracellular Mycobacterium tuberculosis.- Transposon Mutagenesis in Mycobacteria Using Conditionally Replicating Mycobacteriophages.- Gene Replacement and Transposon Delivery Using the Negative Selection Marker sacB.- Gene Replacement using Pretreated DNA.- Gene Replacement in Mycobacterium tuberculosis and Mycobacterium bovis BCG Using rpsL as a Dominant Negative Selectable Marker.- Transcription Start-Site Mapping.- Fluorescence and Brightfield Cytology of Live M. tuberculosis Cells.- Phage Replication Technology for Diagnosis and Drug Susceptibility Testing.- Detection of Mutations in Mycobacterium tuberculosis by a Dot Blot Hybridization Strategy.- Restriction Fragment Length Polymorphism Typing of Mycobacteria.- Preparation of Culture Filtrate Proteins from Mycobacterium tuberculosis.- Analysis of the Capsule of Mycobacterium tuberculosis.- Analysis of the Lipids of Mycobacterium tuberculosis.- In Vitro Model of Hypoxically Induced Nonreplicating Persistence of Mycobacterium tuberculosis.- 17 Macrophage Virulence Assays.- Analysis of Mycobacterium-Infected Macrophages by Immunoelectron Microscopy and Cell Fractionation.- Real Time PCR Using Molecular Beacons.- Electronic Access to Mycobacterium tuberculosis Sequence Data.- Proteomics.- Functional Genomics of Mycobacterium tuberculosis Using DNA Microarrays.- Storage of Mycobacterial Strains.- Safety in the Laboratory.

Summary

The aim of this book is to provide detailed protocols for studying the molecular biology of the pathogen Mycobacterium tuberculosis, and its int- actions with host cells. As established mycobacterial laboratories move - wards exploiting the genome, and laboratories with expertise in other fields apply them to mycobacteria, both traditional and novel methodologies need to be reviewed. Thus the chapters in Mycobacterium tuberculosis Protocols range from perspectives on storage of strains and safety issues to the application of the latest functional genomics technologies. The last few years have been remarkable ones for research into M. tuber- losis. The most important landmark by far has been the completion of the genome sequence of the widely studied H37Rv strain (1). We can now predict every protein and RNA molecule made by the pathogen. This information is or will soon be enriched by the addition of genome sequences of other strains from the M. tuberculosis complex: a second strain of M. tuberculosis, My- bacterium bovis, and the vaccine strain, M. bovis BCG. Valuable comparative data will also be provided by the genome sequences of Mycobacterium leprae, Mycobacterium avium, and Streptomyces coelicolor. Another recent milestone for M. tuberculosis has been the development of efficient mutagenesis me- odologies, the lack of which has been a major handicap in functional studies.

Additional text

...a very useful book for the mycobacteria research community. It is a specialist's cookbook, perfect if you want to establish a new method that is outside your scope of expertise. Individual chapters are clearly divided into introduction, materials, methods and an extensive list of notes. -- International Journal of Medical Microbiology

"For those engaged in mycobacterial research (and possibly other unrelated genera) which involves genetics, biochemistry and immunology, this textbook is essential and, moreover, I would recommend it as reading material for students undertaking molecular biology experimentation." -Today's Life Science

Report

...a very useful book for the mycobacteria research community. It is a specialist's cookbook, perfect if you want to establish a new method that is outside your scope of expertise. Individual chapters are clearly divided into introduction, materials, methods and an extensive list of notes. -- International Journal of Medical Microbiology

"For those engaged in mycobacterial research (and possibly other unrelated genera) which involves genetics, biochemistry and immunology, this textbook is essential and, moreover, I would recommend it as reading material for students undertaking molecular biology experimentation." -Today's Life Science

Product details

• Assisted by: G Stoker (Editor), G Stoker (Editor), Tany Parish (Editor), Tanya Parish (Editor), Neil G. Stoker (Editor)
• Publisher: Springer, Berlin
• Languages: English
• Product format: Paperback / Softback
• Released: 25.10.2010
• EAN: 9781617371844
• ISBN: 978-1-61737-184-4
• No. of pages: 406
• Weight: 606 g
• Illustrations: XIV, 406 p.
• Series: Methods in Molecular Medicine; Methods in Molecular Medicine
• Subjects: Natural sciences, medicine, IT, technology > Medicine > Clinical medicine; C, Medicine, Diseases, infectious diseases