Read more
This concise, truncated version of Nagy, Varghese and Agarwal's Practical Manual of In Vitro Fertilization is comprised of select clinical chapters for a portable, affordable and up-to-date resource. Gamete Assessment, Selection and Micromanipulation in ART covers a variety of topics, including:
- Human oocyte evaluation
- Sperm evaluation and selection
- Micromanipulators and micromanipulation
- Biopsy procedures on oocytes and embryos
- Molecular insights in IVF
Practical for both clinicians and researchers alike, Gamete Assessment, Selection and Micromanipulation in ART brings together all of the need-to-know information about these important topics in reproductive medicine.
List of contents
Part I: Human Oocyte Evaluation in IVF.- Assessment of Oocyte Quality.- Polarization Microscopy.- Cumulus Cell Gene Expression in Assessment of Oocyte Quality.- Part II: Sperm Evaluation and Selection.- Sperm Assessment: Traditional Approaches and Their Indicative Value.- Sperm Assessment: Novel Approaches and Their Indicative Value.- Intracytoplasmic Morphologically Selected Sperm Injection.- Sperm Testing and ICSI Selection by Hyaluronic Acid Binding: The Hyaluronic Acid-Coated Glass Slide and Petri Dish in Andrology and IVF Laboratories.- Electrophoretic Sperm Separation.- Magnetic-Activated Cell Sorting of Human Spermatozoa.- Polscope-Based Sperm Selection.- Part III. Micromanipulators and Micromanipulation.- Hydraulic Manipulators for ICSI.- Research Instruments Micromanipulators.- Eppendorf Micromanipulator: Setting Up and Operation of Micromanipulators.- The Leica Microsystem IMSI System.- Automated Robotic Intracytoplasmic Sperm Injection.- Oocyte Treatment and Preparation for Microinjection.- Mechanism of Human Oocyte Activation during ICSI and Methodology for Overcoming Low or Failed Fertilization.- Livestock Production via Micromanipulation.- Assisted Hatching in IVF.- Part IV: Biopsy Procedures on Oocytes and Embryos.- Polar Body Biopsy.- Cleavage-Stage Embryo Biopsy.- Embryo Biopsy for PGD: Current Perspective.- Microarrays and CGH for PGD of Chromosome Abnormalities and Gene Defects.- Part V: Molecular Insights.- The Role of Mitochondria in the Establishment of Developmental Competence in Early Human Development.- Nuclear and Cytoplasmic Transfer: Human Applications and Concerns.- Cytoskeletal Architecture of Human Oocytes with Focus on Centrosomes and Their Significant Role in Fertilization.- Molecular Mining of Follicular Fluid for Reliable Biomarkers of Human Oocyte and Embryo Developmental Competence.
About the author
Zsolt Peter Nagy, MD, PhD, HCLD (ABB), EMB (ACE), Scientific and Laboratory Director, Reproductive Biology Associates, Atlanta, GA, USA
Alex C. Varghese, PhD, Senior Embryologist, Montreal Reproductive Centre, Montreal, QC, Canada
Ashok Agarwal, PhD, HCLD (ABB), EMB (ACE), Director, Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA
Summary
This concise, truncated version of Nagy, Varghese and Agarwal's
Practical Manual of In Vitro Fertilization
is comprised of select clinical chapters for a portable, affordable and up-to-date resource.
Gamete Assessment, Selection and Micromanipulation in ART
covers a variety of topics, including:
- Human oocyte evaluation
- Sperm evaluation and selection
- Micromanipulators and micromanipulation
- Biopsy procedures on oocytes and embryos
- Molecular insights in IVF
Practical for both clinicians and researchers alike,
Gamete Assessment, Selection and Micromanipulation in ART
brings together all of the need-to-know information about these important topics in reproductive medicine.
