Bacterial Pathogenesis - Methods and Protocols

Bacterial infections affect world health today as a leading cause of morbidity and mortality. Pathogenic bacteria routinely command a broad spectrum of niches in the human host, making an understanding of pathogenesis mechanisms crucial to the development of prophylactics and treatment for bacterial diseases. A variety of in vitro methods, in vivo animal model systems and cutting-edge genomics assays have arisen in the effort to study bacterial pathogenesis and identify potential therapeutic targets. In Bacterial Pathogenesis, in-depth methods and state-of-the-art protocols are presented for investigating specific mechanisms of pathogenesis for a wide range of bacteria. This invaluable collection includes protocols to study host-pathogen interactions, animal models of infection, and novel approaches to identifying therapeutic targets designed to control infections. Up-to-date molecular typing methods for Staphylococcus aureus and a new model of streptococcal pharyngitis in non-human primates are also included. Bacterial Pathogenesis will prove an invaluable collection for microbiologists, immunologists, cell biologists and infectious disease clinicians - and indispensable to all science researchers interested in studying pathogenic bacteria and related disease processes.

List of contents

Preface.- Contributors.- List of Color Plates.- I: Mechanisms of Bacterial Pathogenesis.- 1 Isolation and Characterization of LipopolysaccharidesMichael A. Apicella.- 2 Proteomic Analysis of Proteins Secreted by Streptococcus pyogenesMichelle A. Chaussee, Emily J. McDowell, and Michael S. Chaussee.- 3 Proteomic Analysis to Investigate Regulatory Networks in Staphylococcus aureusSusanne Engelmann and Michael Hecker.- 4 Microarray Comparative Genomic Hybridization for the Analysis of Bacterial Population Genetics and EvolutionCaitriona M. Guinane and J. Ross Fitzgerald.- 5 Detection and Inhibition of Bacterial Cell-Cell CommunicationScott A. Rice, Diane McDougald, Michael Givskov, and Staffan Kjelleberg.- 6 A System for Site-Specific Genetic Manipulation of the Relapsing Fever Spirochete Borrelia hermsiiJames M. Battisti, Sandra J. Raffel, and Tom G. Schwan.- 7 Transposon Mutagenesis of the Lyme Disease Agent Borrelia burgdorferiPhilip E. Stewart and Patricia A. Rosa.- 8 The Biofilm Exopolysaccharide Polysaccharide Intercellular Adhesin?A Molecular and Biochemical ApproachCuong Vuong and Michael Otto.- II: Host-Pathogen Interaction.- 9 Analysis of Staphylococcus aureus Gene Expression During PMN PhagocytosisJovanka M. Voyich, Dan E. Sturdevant, and Frank R. DeLeo.- 10 Examining the Vector-Host-Pathogen Interface With Quantitative Molecular ToolsJason E. Comer, Ellen A. Lorange, and B. Joseph Hinnebusch.- 11 Intracellular Localization of Brucella abortus and Francisella tularensis in Primary Murine MacrophagesJean Celli.- 12 Rate and Extent of Helicobacter pylori PhagocytosisLee-Ann H. Allen.- 13 Culture, Isolation, and Labeling of Anaplasma phagocytophilum for Subsequent Infection of Human NeutrophilsDori L. Borjesson.- 14 Ultrastructural Analysis ofBacteria-Host Cell InteractionsDavid W. Dorward.- 15 Infection of Human Monocyte-Derived Macrophages With Coxiella burnetiiJeffrey G. Shannon and Robert A. Heinzen.- 16 Infection of Epithelial Cells With Salmonella entericaOlivia Steele-Mortimer.- 17 Determining the Cellular Targets of Reactive Oxygen Species in Borrelia burgdorferiJulie A. Boylan and Frank C. Gherardini.- III: Animal Models of Bacterial Infection.- 18 Bioluminescent Imaging of Bacterial Biofilm Infections In VivoJagath L. Kadurugamuwa and Kevin P. Francis.- 19 The Cotton Rat as a Model for Staphylococcus aureus Nasal Colonization in Humans: Cotton Rat S. aureus Nasal Colonization ModelJohn F. Kokai-Kun.- 20 A Non-Human Primate Model of Acute Group A Streptococcus PharyngitisPaul Sumby, Anne H. Tart, and James M. Musser.- IV: Identification of Therapeutic Targets and Typing Methods.- 21 Target-Based Antimicrobial Drug DiscoveryLefa E. Alksne and Paul M. Dunman.- 21 Sequence Analysis of the Variable Number Tandem Repeat in Staphylococcus aureus Protein A Gene: spa TypingBarun Mathema, Jose Mediavilla, and Barry N. Kreiswirth.

Summary

Bacterial infections affect world health today as a leading cause of morbidity and mortality. Pathogenic bacteria routinely command a broad spectrum of niches in the human host, making an understanding of pathogenesis mechanisms crucial to the development of prophylactics and treatment for bacterial diseases. A variety of in vitro methods, in vivo animal model systems and cutting-edge genomics assays have arisen in the effort to study bacterial pathogenesis and identify potential therapeutic targets. In Bacterial Pathogenesis, in-depth methods and state-of-the-art protocols are presented for investigating specific mechanisms of pathogenesis for a wide range of bacteria. This invaluable collection includes protocols to study host-pathogen interactions, animal models of infection, and novel approaches to identifying therapeutic targets designed to control infections. Up-to-date molecular typing methods for Staphylococcus aureus and a new model of streptococcal pharyngitis in non-human primates are also included. Bacterial Pathogenesis will prove an invaluable collection for microbiologists, immunologists, cell biologists and infectious disease clinicians - and indispensable to all science researchers interested in studying pathogenic bacteria and related disease processes.

Additional text

From the reviews:

"An eclectic collection of papers ranges from those with limited general applications, to those with a much broader interest. … The additional notes in each paper are most useful, and may help those making use of these techniques avoid some of the frustrations associated with following methodologies in published journal papers. … a volume to dip into seeking inspiration for new techniques to apply to one’s own favourite organism." (Jonathan Fletcher, Microbiology Today, July, 2008)

"This book was written to bring scientists working in the field of bacterial pathogenesis up-to-date with both traditional tried-and-tested methods that have been in use for decades, and more recent technological advances and breakthroughs that have led to the development of new protocols. … On the whole this book is very informative and includes an extensive set of complex and detailed protocols. … I would recommend to anyone in the field or thinking of entering the field of bacterial pathogenesis." (Andrew Vaughan, Immunology News, August, 2008)

"The 431st volume of Methods in Molecular Biology published by Humana Press is dedicated to the problems of bacterial pathogenesis. ... Each chapter is concluded by an exhaustive list of references. ... By its high level of interest and schematic approach the book is suitable not only for scientists dealing with bacterial pathogenesis ... but it can be recommended also for graduate students in microbiology who are about to start their scientific career." (K. Tyllovâ, Folia Microbiologica, Vol. 53, November, 2008)

Report

From the reviews:

"An eclectic collection of papers ranges from those with limited general applications, to those with a much broader interest. ... The additional notes in each paper are most useful, and may help those making use of these techniques avoid some of the frustrations associated with following methodologies in published journal papers. ... a volume to dip into seeking inspiration for new techniques to apply to one's own favourite organism." (Jonathan Fletcher, Microbiology Today, July, 2008)
"This book was written to bring scientists working in the field of bacterial pathogenesis up-to-date with both traditional tried-and-tested methods that have been in use for decades, and more recent technological advances and breakthroughs that have led to the development of new protocols. ... On the whole this book is very informative and includes an extensive set of complex and detailed protocols. ... I would recommend to anyone in the field or thinking of entering the field of bacterial pathogenesis." (Andrew Vaughan, Immunology News, August, 2008)
"The 431st volume of Methods in Molecular Biology published by Humana Press is dedicated to the problems of bacterial pathogenesis. ... Each chapter is concluded by an exhaustive list of references. ... By its high level of interest and schematic approach the book is suitable not only for scientists dealing with bacterial pathogenesis ... but it can be recommended also for graduate students in microbiology who are about to start their scientific career." (K. Tyllovâ, Folia Microbiologica, Vol. 53, November, 2008)

Product details

• Assisted by: Fran DeLeo (Editor), Frank Deleo (Editor), Frank R. DeLeo (Editor), Otto (Editor), Otto (Editor), Michael Otto (Editor)
• Publisher: Springer, Berlin
• Languages: English
• Product format: Paperback / Softback
• Released: 26.10.2010
• EAN: 9781617377518
• ISBN: 978-1-61737-751-8
• No. of pages: 320
• Dimensions: 152 mm x 17 mm x 229 mm
• Weight: 477 g
• Illustrations: XVI, 320 p. 47 illus., 10 illus. in color.
• Series: Methods in Molecular Biology; Methods in Molecular Biology
• Subjects: Natural sciences, medicine, IT, technology > Biology > General, dictionaries; B, Immunology, Microbiology (non-medical), microbiology, Biomedical and Life Sciences, Cellular biology (cytology), Medical Microbiology, Cell Biology