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After decades of research in clinical transplantation, new techniques have been developed that permit a further understanding of the immune mechanisms underlying immune recognition of allografts and a more accurate and thorough evaluation of compatibility between donors and recipients. The second edition of Transplantation Immunology: Methods and Protocols expands upon the previous edition with current, detailed methods in transplantation immunology. The new methods chapters cover four major areas that are being applied in compatibility evaluations and ongoing transplantation immunology research. Seven overview chapters provide reviews of the molecular basis for alloreactivity, current understanding of humoral and cellular mechanisms, as well as new developments in thoracic organ transplantation, composite tissue transplantation and in the transplantation of sensitized patients. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Transplantation Immunology: Methods and Protocol, Second Edition is devoted to transplantation immunology, both in the practice of compatibility testing and in transplantation research.
List of contents
Alloreactivity.- Antibodies in Transplantation: The Effects of HLA and Non-HLA Antibody
Binding and Mechanisms of Injury.- Cell Mediated Rejection.- Transplantation Tolerance.- Composite Tissue Transplantation.- Transplantation of the Sensitized Patient: Histocompatibility Testing.- Thoracic Organ Transplantation: Laboratory Methods.- HLA Typing by Sequence Specific Primers.- Human Leukocyte Antigen (HLA) Typing By DNA Sequencing.- Next-Generation HLA Sequencing using the 454 GS FLX System.- Chimerism Testing by Quantitative PCR Using Indel Markers.- Gene-specific PCR Typing of Killer Cell Immunoglobulin-like Receptors (KIR).- Complement-dependent Cytotoxicity Crossmatch.- Lymphocyte Crossmatching by Flow Cytometry.- Solid Phase Assay Measuring C4d Deposition to Determine Complement Fixation by HLA Specific Antibodies.- C1q Assay for the Detection of Complement Fixing Antibody to HLA Antigens.- Tetramer Staining for the Detection of HLA-specific B cells.- A Flow Cytometric Crossmatch Test Using Endothelial Precursor Cells Isolated from Peripheral Blood.- Detection of Antibodies to Self-antigens (K-alpha 1 Tubulin, Collagen I, II, IV and V Myosin and Vimentin) by Enzyme-Linked Immunosorbent Assay (ELISA).- Cylex ImmuKnow Cell Function Assay.- Detection of Intracellular Cytokines.- Tolerogenic Dendritic Cells and Induction of T Suppresson Cells in Transplant Recipients.- Discovery and Customized Validation of Antibody Targets by Protein Arrays and Indirect ELISA.- RNA Purification and Expression Analysis using Microarrays and RNA Deep Sequencing.
Summary
After decades of research in clinical transplantation, new techniques have been developed that permit a further understanding of the immune mechanisms underlying immune recognition of allografts and a more accurate and thorough evaluation of compatibility between donors and recipients. The second edition of Transplantation Immunology: Methods and Protocols expands upon the previous edition with current, detailed methods in transplantation immunology. The new methods chapters cover four major areas that are being applied in compatibility evaluations and ongoing transplantation immunology research. Seven overview chapters provide reviews of the molecular basis for alloreactivity, current understanding of humoral and cellular mechanisms, as well as new developments in thoracic organ transplantation, composite tissue transplantation and in the transplantation of sensitized patients. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Transplantation Immunology: Methods and Protocol, Second Edition is devoted to transplantation immunology, both in the practice of compatibility testing and in transplantation research.
Additional text
From the reviews of the second edition:
“The purpose is to provide an overview of key areas of research in transplant immunology and detailed descriptions of relevant methodology. An appropriate audience includes solid organ transplant physicians, immunologists, and immunology laboratory staff. … I find this book very useful. It covers important topics in transplant immunology in great detail, and is a wonderful opportunity to review the current understanding of important topics by leaders in the field.” (Christina Klein, Doody’s Book Reviews, November, 2013)
Report
From the reviews of the second edition:
"The purpose is to provide an overview of key areas of research in transplant immunology and detailed descriptions of relevant methodology. An appropriate audience includes solid organ transplant physicians, immunologists, and immunology laboratory staff. ... I find this book very useful. It covers important topics in transplant immunology in great detail, and is a wonderful opportunity to review the current understanding of important topics by leaders in the field." (Christina Klein, Doody's Book Reviews, November, 2013)
