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Klappentext Proteins Biochemistry and Biotechnology 2e is a definitive source of information for all those interested in protein science! and particularly the commercial production and isolation of specific proteins! and their subsequent utilization for applied purposes in industry and medicine. Zusammenfassung Proteins Biochemistry and Biotechnology 2e is a definitive source of information for all those interested in protein science! and particularly the commercial production and isolation of specific proteins! and their subsequent utilization for applied purposes in industry and medicine. Preface xi About the Companion Website xiii Chapter 1 Proteins and proteomics 1 1.1 Proteins, an introduction 1 1.2 Genes, genomics and proteomics 2 1.3 Bioinformatics 12 1.4 Proteomics: goals and applications 14 Further reading 22 Chapter 2 Protein structure and engineering 25 2.1 Primary structure 25 2.2 Higher-level structure 36 2.3 Protein classification on the basis of structure 41 2.4 Protein structural stability 45 2.5 Higher-order structure prediction 47 2.6 Protein folding 48 2.7 Intrinsically disordered proteins 50 2.8 Protein engineering 51 2.9 Protein post-translational modification 54 Further reading 62 Chapter 3 Protein sources 65 3.1 Recombinant versus non-recombinant production 65 3.2 Approaches to recombinant protein production 67 3.3 Heterologous protein production in E. coli 72 3.4 Heterologous production in bacteria other than E. coli 77 3.5 Heterologous protein production in yeast 77 3.6 Heterologous protein production in fungi 78 3.7 Proteins from plants 80 3.8 Animal tissue as a protein source 84 3.9 Heterologous protein production in transgenic animals 85 3.10 Heterologous protein production using animal cell culture 86 3.11 Insect cell culture systems 87 Chapter 4 Protein purification and characterization 91 4.1 Protein detection and quantification 93 4.2 Initial recovery of protein 95 4.3 Removal of whole cells and cell debris 98 4.4 Concentration 103 4.5 Chromatographic purification 107 4.6 Protein inactivation and stabilization 128 4.7 Protein characterization 137 Further reading 139 Chapter 5 Large-scale protein production 141 5.1 Upstream processing 141 5.2 Downstream processing 154 5.3 Therapeutic protein production: some special issues 163 5.4 Range and medical significance of impurities potentially present in protein-based therapeutic products 166 Further reading 175 Chapter 6 Therapeutic proteins: blood products, vaccines and enzymes 177 6.1 Blood products 177 6.2 Anticoagulants 184 6.3 Thrombolytic agents 186 6.4 Additional blood-related products 189 6.5 Vaccine technology 190 6.6 Therapeutic enzymes 194 Further reading 202 Chapter 7 Therapeutic antibodies 205 7.1 Antibodies 205 7.2 IgG structure and activity 205 7.3 Antibody therapeutics: polyclonal antibody preparations 209 7.4 Antibody therapeutics: monoclonal antibodies 211 7.5 Therapeutic applications of monoclonal antibodies 220 7.6 Antibody conjugates 223 7.7 Bispecific antibodies 224 7.8 Antibody fragments 225 7.9 Engineering the antibody glycocomponent 228 7.10 Fc fusion proteins 229 Further reading 230 Chapter 8 Hormones and growth factors used therapeutically 233 8.1 Insulin 233 8.2 Glucagon 240 8.3 Gonadotrophins 240 8.4 Growth hormone 243 8.5 Erythropoietin 246 8.6 Other hormones 247 8.7 Growth factors 249 Further reading 253 Chapter 9 Interferons, interleukins and tumour necrosis factors 257 9.1 Regulatory factors: cytokines versus hormones 257 9.2 Interferons 258 9.3 Interleukins 264 9.4 Tumour necrosis factors 271 Further reading 274 Chapter 10 Proteins used for analytical purposes 277 10.1 The IVD sector 279 10.2 The basis of analyte detection and quantification 280 10.3 Enzymes as diagnostic/analytical reagents 281 10.4 Biosensors 289 10.5 Antibodies as analytical reagents 295 Further readin...
