Capillary Electrophoresis and Microchip Capillary Electrophoresis - Principles, Applications, and Limitations

Informationen zum Autor Carlos D. García, PhD, is an Associate Professor of Analytical Chemistry at the University of Texas at San Antonio, USA. His group is currently focused on the development of novel bioanalytical strategies involving microfluidics and nanomaterials. Karin Y. Chumbimuni-Torres, PhD, is a Research Associate at the University of Texas at San Antonio, USA. She is interested in pursuing the development of electrochemical biosensors and their integration to microchip-based platforms. Emanuel Carrilho, PhD, is an Associate Professor at the University of Säo Paulo, Brazil. With more than twenty-five years of experience in separation science, his group is focused on the development of analytical methods and instrumentation for bioanalyses. Klappentext Explores the benefits and limitations of the latest capillary electrophoresis techniquesCapillary electrophoresis and microchip capillary electrophoresis are powerful analytical tools that are particularly suited for separating and analyzing biomolecules. In comparison with traditional analytical techniques, capillary electrophoresis and microchip capillary electrophoresis offer the benefits of speed, small sample and solvent consumption, low cost, and the possibility of miniaturization.With contributions from a team of leading analytical scientists, Capillary Electrophoresis and Microchip Capillary Electrophoresis explains how researchers can take full advantage of all the latest techniques, emphasizing applications in which capillary electrophoresis has proven superiority over other analytical approaches. The authors not only explore the benefits of each technique, but also the limitations, enabling readers to choose the most appropriate technique to analyze a particular sample.The book's twenty-one chapters explore fundamental aspects of electrophoretically driven separations, instrumentation, sampling techniques, separation modes, detection systems, optimization strategies for method development, and applications. Specific topics include:* Critical evaluation of the use of surfactants in capillary electrophoresis* Sampling and quantitative analysis in capillary electrophoresis* Capillary electrophoresis with electrochemical detection* Overcoming challenges in using microchip electrophoresis for extended monitoring applications* Capillary electrophoresis of intact unfractionated heparin and related impurities* Microchip capillary electrophoresis for in situ planetary explorationEach chapter begins with an introduction and ends with conclusions as well as references to the primary literature. Novices to the field will find this book an easy-to-follow introduction to core capillary electrophoresis techniques and methods. More experienced investigators can turn to the book for troubleshooting tips and expert advice to guide them through the most advanced applications. Zusammenfassung Providing the most current information related to separations by capillary electrophoresis and microchip capillary electrophoresis, this innovative text provides a fundamental understanding of the CE and microchip-CE and their applications, along with troubleshooting hints. Inhaltsverzeichnis PREFACE xvii ACKNOWLEDGMENTS xix CONTRIBUTORS xxi 1 Critical Evaluation of the Use of Surfactants in Capillary Electrophoresis 1 Jessica L. Felhofer, Karin Y. Chumbimuni-Torres, Maria F. Mora, Gabrielle G. Haby, and Carlos D. Garcý¿a 1.1 Introduction 1 1.2 Surfactants for Wall Coatings 4 1.2.1 Controlling the Electroosmotic Flow 4 1.2.2 Preventing Adsorption to the Capillary 5 1.3 Surfactants as Buffer Additives 6 1.3.1 Micellar Electrokinetic Chromatography 6 1.3.2 Microemulsion Electrokinetic Chromatography 8 1.3.3 Nonaqueous Capillary Electrophoresis with Added Surfactants 9 1.4 Surfactants for Anal...

List of contents

PREFACE xvii
 
ACKNOWLEDGMENTS xix
 
CONTRIBUTORS xxi
 
1 Critical Evaluation of the Use of Surfactants in Capillary Electrophoresis 1
Jessica L. Felhofer, Karin Y. Chumbimuni-Torres, Maria F. Mora, Gabrielle G. Haby, and Carlos D. Garc?´a
 
1.1 Introduction 1
 
1.2 Surfactants for Wall Coatings 4
 
1.3 Surfactants as Buffer Additives 6
 
1.4 Surfactants for Analyte Preconcentration 9
 
1.5 Surfactants and Detection in CE 14
 
1.6 Conclusions 16
 
References 17
 
2 Sample Stacking: A Versatile Approach for Analyte Enrichment in CE and Microchip-CE 23
Bruno Perlatti, Emanuel Carrilho, and Fernando Armani Aguiar
 
2.1 Introduction 23
 
2.2 Isotachophoresis 24
 
2.3 Chromatography-Based Sample Stacking 25
 
2.4 Methods Based on Electrophoretic Mobility and Velocity Manipulation (Electrophoretic Methods) 26
 
2.5 Sample Stacking in Pseudo-Stationary Phases 29
 
2.6 Stacking Techniques in Microchips 33
 
2.7 Concluding Remarks 36
 
References 37
 
3 Sampling and Quantitative Analysis in Capillary Electrophoresis 41
Petr Kuba´9n, Andrus Seiman, and Mihkel Kaljurand
 
3.1 Introduction 41
 
3.2 Injection Techniques in CE 42
 
3.3 Micromachined/Microchip Injection Devices 53
 
3.4 Automated Flow Sample Injection and Hyphenated Systems 55
 
3.5 Computerized Sampling and Data Analysis 57
 
3.6 Sampling in Portable CE Instrumentation 58
 
3.7 Quantitative Analysis in CE 59
 
3.8 Conclusions 62
 
References 62
 
4 Practical Considerations for the Design and Implementation of High-Voltage Power Supplies for Capillary and Microchip Capillary Electrophoresis 67
Lucas Blanes, Wendell Karlos Tomazelli Coltro, Renata Mayumi Saito, Claudimir Lucio do Lago, Claude Roux, and Philip Doble
 
4.1 Introduction 67
 
4.2 High-Voltage Measurement 73
 
4.3 Concluding Remarks 74
 
References 74
 
5 Artificial Neural Networks in Capillary Electrophoresis 77
Josef Havel, Eladia Mar?a Pe~na-Mendez, and Alberto Rojas-Hernandez
 
5.1 Introduction 77
 
5.2 Optimization in CE: From Single Variable Approach Toward Artificial Neural Networks 77
 
5.3 Artificial Neural Networks in Electromigration Methods 81
 
5.4 Conclusions 90
 
Acknowledgments 91
 
References 91
 
6 Improving the Separation in Microchip Electrophoresis by Surface Modification 95
M. Teresa Fernandez-Abedul, Isabel Alvarez-Martos, Francisco Javier Garc?a Alonso, and Agust?n Costa-Garc?a
 
6.1 Introduction 95
 
6.2 Strategies for Improving Separation 96
 
6.3 Chemical Modifiers 102
 
6.4 Conclusions 119
 
Acknowledgments 120
 
References 120
 
7 Capillary Electrophoretic Reactor and Microchip Capillary Electrophoretic Reactor: Dissociation Kinetic Analysis Method for "Complexes" Using Capillary Electrophoretic Separation Process 127
Toru Takahashi and Nobuhiko Iki
 
7.1 Introduction 127
 
7.2 Basic Concept of CER 128
 
7.3 Dissociation Kinetic Analysis of Metal Complexes Using a CER 129
 
7.4 Expanding the Scope of the CER to Measurements of Fast Dissociation Kinetics with a Half-Life from Seconds to Dozens of Seconds: Dissociation Kinetic Analysis of Metal Complexes Using a Microchip Capillary Electrophoretic Reactor (mCER) 133
 
7.5 Expanding the Scope of the CER to the Measurement of Slow Dissociation Kinetics with a Half-Life of Hours 135
 
7.6 Expanding the Scope of CER to Measurement of the Dissociation Kinetics of Biomolecular Complexes 139
 
7.7 Conclusions 142
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Product details

• Authors: Carril, Emanuel Carrilho, Karin Chumbimuni-Torres, Karin Y Chumbimuni-Torres, Karin Y. Chumbimuni-Torres, Chumbimuni-Torres Karin Y., Carlos D. García, Carlos D. Garc?a, Carlos D. Garcia, Carlos D. Chumbimuni-Torres Garcia, CD Garcia, Carlos García, Carlos D García, Carlos D. García, GARCIA CARLOS D CHUMBIMUNI TORRE
• Publisher: Wiley, John and Sons Ltd
• Languages: English
• Product format: Hardback
• Released: 12.04.2013
• EAN: 9780470572177
• ISBN: 978-0-470-57217-7
• No. of pages: 416
• Subjects: Natural sciences, medicine, IT, technology > Chemistry > Organic chemistry; Chemie, Analytische Chemie, Life Sciences, Biowissenschaften, chemistry, Analytical Chemistry, Pharmaceutical & Medicinal Chemistry, Pharmazeutische u. Medizinische Chemie, Genomforschung u. Proteomik, Genomics & Proteomics