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Genetic engineering through DNA recombinants and the in vitro manipulation of isolated protoplasts has recently attracted much atten tion in agricultural biotechnology, and has greatly advanced during the last 5 years. In an earlier book, Plant Protoplasts and Genetic Engineer ing I, methods for the isolation, fusion and culture of protoplasts were reviewed and the regeneration of complete plants from isolated pro toplasts of rice, potato, soybean, linseed, cabbage, chicory, lettuce, but terbur, orchids, citrus and some other tree species, and interspecific and intergeneric somatic hybrids in Lycopersicon, Petunia, Nicotiana, Solanum, Glycine, Citrus, Brassica, Medicago and Trifolium spp. were discussed. The present volume, Plant Protoplasts and Genetic Engineering II, deals with some of the newer techniques such as microinjections, elec trofusion, flow cytometry, uptake and integration of DNA, nuclei, iso lated chromosomes by plant protoplasts and the subsequent regeneration of transgenic plants. The literature on the DNA recombinants and genetic transformation, both Agrobacterium-mediated and direct gene transfer in agricultural crops and trees, such as poplars, is reviewed, and the uses of cytoplasts and miniprotoplasts in genetic manipulation are highlighted.
List of contents
I Genetic Engineering and In Vitro Manipulation of Plant Cells - Technical Advances.- II Genetic Manipulation: Generative Versus Somatic.- III Uptake and Integration of Exogenous DNA in Plants.- IV DNA Recombinants and Transformation of Agricultural Crops.- V Genetically Transformed Maize (Zea mays L.) Plants from Protoplasts.- VI Protoplast Culture and Transformation Studies on Rice.- VII Transformation in Potato.- VIII Transformation in Cotton.- IX Transformation in Populus spp..- X Transformation in Vinca rosea L.: An Example of Spheroplast Method for Gene Transfer.- XI Transformation in Hyoscyamus.- XII Genetic Manipulation in Brassica.- XIII Direct Gene Transfer in Protoplasts of Nicotiana plumbaginifolia.- XIV Microinjection of Cells and Protoplasts: Integration of Foreign DNA.- XV Electrofusion: The Technique and Its Application to Somatic Hybridization.- XVI Versatile Apparatuses for Electrogene Mapping, Electrophoresis and Electrofusion.- XVII Chloroplast Genomes as Genetic Markers.- XVIII Flow Cytometric Analysis and Sorting of Somatic Hybrid and Transformed Protoplasts.- XIX Isolation of Nuclei and Their Transplantation into Plant Protoplasts.- XX Chromosome Transplantation and Applications of Flow Cytometry in Plants.- XXI Transfer of Mitochondria Through Protoplast Fusion.- XXII Incorporation of the Firefly Luciferase Gene into Plant Cells.- XXIII Uptake of Viruses by Plant Protoplasts and Their Use as Transforming Agents.- XXIV Uptake of Fungal Protoplasts by Plant Protoplasts.- XXV Chloroplast Uptake and Genetic Complementation.- XXVI Cytoplasts: Isolation and Uses.- XXVII Vacuoles: Isolation, Purification, and Uses.- XXVIII Miniprotoplasts and Their Use in Genetic Engineering.
