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Modern cell biology is being revolutionized by the wedding of microscopy and computers. This book describes the new instrumentation and methods which allow three-dimensional reconstruction of specimens. Multidimensional Microscopy will be of interest to cell biologists, microscopists, and basic biomedical researchers whose work involves microscopic techniques. This book presents current results on a very active field in modern biology: methods in light and electron microscopy that allow the reconstruction of three-dimensional objects with the aid of computers. The book emphasizes the methods that can be used and examples of biological systems to which they have been applied. It includes extensive descriptions of confocal microscopy and its applications, as well as chapters on X-ray microscopy, low-voltage electron microscopy, and image reconstruction. This is an impressive summary of state-of-the-art methods in microscopy, in which microscopes and computers are being joined to permit specimens to be examined and reconstructed in three dimensions. Will be of interest to cell biologists, biomedical researchers, and microscopists.
List of contents
1 Confocal Microscopy: Basic Principles and System Performance.- 2 Designing a Confocal Fluorescence Microscope.- 3 Theory and Principal Technologies of the Laser Scanning Confocal Microscope.- 4 Simultaneous Ultraviolet and Visible Excitation Confocal Microscopy.- 5 High Resolution Confocal Microscopy of Phase and Amplitude Objects.- 6 Interferometric Microscopy - An Overview of the Optical Characterization of Inertial-fusion Targets.- 7 X-Ray Microscopy - Possibilities for Three-Dimensional Imaging.- 8 High Resolution Projection X-Ray Microscopy.- 9 Cone-beam X-ray Microtomography.- 10 High Resolution Low Voltage Scanning Electron Microscopy: Reduced Radiation Damage on Cryo-specimens.- 11 Image Restoration in Light Microscopy.- 12 Image Quality Considerations in Computerized 2-D and 3-D Microscopy.- 13 Analysis and Presentation of Three-Dimensional Data Sets.- 14 The Processing of Three-Dimensional Confocal Data Sets.- 15 Effective Laser Lines for Biological Confocal Microscopy.- 16 Visualizing DNA Replication in Three Dimensions.- 17 Study of Nuclear Proteins Associated with Cell Proliferation, Differentiation, and Mitosis by Confocal Fluorescence Microscopy.- 18 Two- and Three-Dimensional Confocal Microscopy of the Eye.- 19 Confocal Microscopy of Botanical Specimens.
