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Plant Cryopreservation: A Practical Guide is a unique resource for plant scientists in many fields. This compilation provides over 100 ready-to-use protocols for plant types from algae and bryophytes to a range of flowering plants. It includes techniques for diverse plant parts such as dormant buds, pollen, and apical meristems, and for cell types such as suspension and callus cultures. Five introductory chapters describe theoretical principles, practical aspects of long-term cryopreserved storage, and details of the main cryopreservation techniques. The remaining 14 chapters are separated by plant type. Each chapter briefly reviews the literature and includes ready-to-use protocols designed for easy transfer into the lab and adaptation to new species.
List of contents
to Cryopreservation.- Cryopreservation-Practical Considerations.- Cryopreservation Theory.- Development of PVS-Based Vitrification and Encapsulation-Vitrification Protocols.- Development of Encapsulation Dehydration.- Controlled Rate Cooling.- Cryopreservation Protocols for Working Laboratories.- Cryopreservation of Algae.- Cryopreservation of Bryophytes and Ferns.- Cryopreservation of Dedifferentiated Cell Cultures.- Cryopreservation of Embryogenic Cultures.- Cryopreservation of Excised Embryos and Embryonic Axes.- Cryopreservation of Monocots.- Cryopreservation of Herbaceous Dicots.- Cryopreservation of Temperate Berry Crops.- Cryopreservation of In Vitro Tissues of Deciduous Forest Trees.- Cryopreservation of Fruit and Ornamental Trees.- Cryopreservation of Dormant Buds.- Cryopreservation of Pollen.- Cryopreservation of Recalcitrant (i.e. Desiccation-Sensitive) Seeds.- Cryopreservation of Orthodox (Desiccation Tolerant) Seeds.
Summary
Plant Cryopreservation: A Practical Guide is a unique resource for plant scientists in many fields. This compilation provides over 100 ready-to-use protocols for plant types from algae and bryophytes to a range of flowering plants. It includes techniques for diverse plant parts such as dormant buds, pollen, and apical meristems, and for cell types such as suspension and callus cultures. Five introductory chapters describe theoretical principles, practical aspects of long-term cryopreserved storage, and details of the main cryopreservation techniques. The remaining 14 chapters are separated by plant type. Each chapter briefly reviews the literature and includes ready-to-use protocols designed for easy transfer into the lab and adaptation to new species.
