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Klappentext In the last two decades, a remarkable series of improvements has occurred in the design of optical microscopes, in concert with developments in post-acquisition image processing and enabled by the enormous advances in computer power. The outcome of these developments is a steady and dramatic improvement in resolution. The original concept, as laid out by Ernst Abbe in the 1890s, was that resolution in optical microscopy has its limit, and as a consequence many investigators took for granted that optical microscopy had reached its final stage. Thanks to the efforts by many researchers exploring new avenues in optical microscopy, we can say today that nothing in the optical realm should be taken for granted and that Abbe's refraction barrier, considered by so many for so long as a near-absolute barrier, has been widely breached. Thus the gap between optical and electron microscopy is being closed by far-reaching developments in optical microscopy. Methods have progressed alongside the technological advances, and innovative ideas and procedures have been developed to bridge the resolution gap from the electron microscope level, by performing correlative light and electron microscopy. In Cellular Imaging Techniques for Neuroscience and Beyond, specialists report on the current status of various approaches nibbling away at Abbe's barrier. Zusammenfassung The imaging of small cellular components requires powerful instruments! and an entire family of equipment and techniques based on the confocal principle has been developed over years. This book discusses developments for the future and aids readers in selecting the scientifically meaningful approach to solve their questions. Inhaltsverzeichnis 1. Confocal laser scanning: Of instrument, computer processing, and men Jeroen A.M. Beliën and Floris G. Wouterlood 2. Beyond Abbe's resolution barrier: STED microscopy U. Valentin Nägerl 3. Enhancement of optical resolution by 4pi single and multiphoton confocal fluorescence microscopy W.A. van Cappellen, A. Nigg, and A.B. Houtsmuller 4. Nano resolution optical imaging through localization microscopy Helge Ewers 5. Optical investigation of brain networks using structured illumination Marco Dal Maschio, Francesco Difato, Riccardo Beltramo, Angela Michela De Stasi, Axel Blau, and Tommaso Fellin 6. Multiphoton microscopy advances toward super resolution Paolo Bianchini, Partha P. Mondal, Shilpa Dilipkumar, Francesca Cella Zanacchi, Emiliano Ronzitti, and Alberto Diaspro 7. The cell at molecular resolution: Principles and applications of cryo-electron tomography Rubén Fernández-Busnadiego and Vladan Lucic 8. Cellular-level optical biopsy using full-field optical coherence microscopy Arnaud Dubois 9. Retroviral labeling and imaging of newborn neurons in the adult brain Kurt A. Sailor, Hongjun Song, and Guo-Li Ming 10. Study of myelin sheaths by CARS microscopy Chun-Rui Hu, Bing Hu, and Ji-Xen Cheng 11. High-resolution approaches to studying presynaptic vesicle dynamics using variants of FRAP and electron microscopy Kevin Staras and Tiago Branco ...
List of contents
1. Confocal laser scanning: Of instrument, computer processing, and men
Jeroen A.M. Beliën and Floris G. Wouterlood
2. Beyond Abbe's resolution barrier: STED microscopy
U. Valentin Nägerl
3. Enhancement of optical resolution by 4pi single and multiphoton confocal fluorescence microscopy
W.A. van Cappellen, A. Nigg, and A.B. Houtsmuller
4. Nano resolution optical imaging through localization microscopy
Helge Ewers
5. Optical investigation of brain networks using structured illumination
Marco Dal Maschio, Francesco Difato, Riccardo Beltramo, Angela Michela De Stasi, Axel Blau, and Tommaso Fellin
6. Multiphoton microscopy advances toward super resolution
Paolo Bianchini, Partha P. Mondal, Shilpa Dilipkumar, Francesca Cella Zanacchi, Emiliano Ronzitti, and Alberto Diaspro
7. The cell at molecular resolution: Principles and applications of cryo-electron tomography
Rubén Fernández-Busnadiego and Vladan Lucic
8. Cellular-level optical biopsy using full-field optical coherence microscopy
Arnaud Dubois
9. Retroviral labeling and imaging of newborn neurons in the adult brain
Kurt A. Sailor, Hongjun Song, and Guo-Li Ming
10. Study of myelin sheaths by CARS microscopy
Chun-Rui Hu, Bing Hu, and Ji-Xen Cheng
11. High-resolution approaches to studying presynaptic vesicle dynamics using variants of FRAP and electron microscopy
Kevin Staras and Tiago Branco
Report
"Wouterlood.introduces the confocal principle which eliminates out-of-focus haze, its components, and relevant equations. International scientists explain the principles and related methods of stimulated emission depletion (SRED), single molecule localization, and coherent anti-Stokes Raman (CARS) microscopy; labeling approaches; preparation of samples for imaging; and applications of, and developments in, this new wave of imaging, e.g., visualization of neuronal networks, DNA, and myelin." --Reference and Research Book News, February 2013
